Cell isolation in open microfluidics: Microfluidic probes integrated with dielectrophoresis

Ayoola T. Brimmo, Anoop Menachery, Mohammad A. Qasaimeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports dielectrophoretic (DEP) based single cell sorting using 3D, hump shaped, micro electrodes designed unto the tip of a 3D printed microfluidic probe (MFP). Multiple humps (75 μm height), integrated in a staggered pattern across the surface of the MFP's tip, are made conductive by gold sputtering, and positioned 20μm above an Indium Tin Oxide (ITO) coated glass slide to form a pin-plate electrode configuration. Using this setup, HeLa cells suspended in DEP media were isolated from the Hydrodynamic Flow Confinement (HFC). Results demonstrated an improvement in cell capture efficiency as the number of aspiration aperture increased.

Original languageEnglish (US)
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages2463-2465
Number of pages3
ISBN (Electronic)9781510897571
StatePublished - 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: Nov 11 2018Nov 15 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume4

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period11/11/1811/15/18

Keywords

  • Dielectrophoresis cell isolation
  • Microfluidic probe

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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