TY - GEN
T1 - Cell isolation in open microfluidics
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
AU - Brimmo, Ayoola T.
AU - Menachery, Anoop
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
PY - 2018
Y1 - 2018
N2 - This paper reports dielectrophoretic (DEP) based single cell sorting using 3D, hump shaped, micro electrodes designed unto the tip of a 3D printed microfluidic probe (MFP). Multiple humps (75 μm height), integrated in a staggered pattern across the surface of the MFP's tip, are made conductive by gold sputtering, and positioned 20μm above an Indium Tin Oxide (ITO) coated glass slide to form a pin-plate electrode configuration. Using this setup, HeLa cells suspended in DEP media were isolated from the Hydrodynamic Flow Confinement (HFC). Results demonstrated an improvement in cell capture efficiency as the number of aspiration aperture increased.
AB - This paper reports dielectrophoretic (DEP) based single cell sorting using 3D, hump shaped, micro electrodes designed unto the tip of a 3D printed microfluidic probe (MFP). Multiple humps (75 μm height), integrated in a staggered pattern across the surface of the MFP's tip, are made conductive by gold sputtering, and positioned 20μm above an Indium Tin Oxide (ITO) coated glass slide to form a pin-plate electrode configuration. Using this setup, HeLa cells suspended in DEP media were isolated from the Hydrodynamic Flow Confinement (HFC). Results demonstrated an improvement in cell capture efficiency as the number of aspiration aperture increased.
KW - Dielectrophoresis cell isolation
KW - Microfluidic probe
UR - http://www.scopus.com/inward/record.url?scp=85066629881&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066629881&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85066629881
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 2463
EP - 2465
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
Y2 - 11 November 2018 through 15 November 2018
ER -