TY - JOUR
T1 - Cell migration along the lateral cortical stream to the developing basal telencephalic limbic system
AU - Carney, Rosalind S.E.
AU - Alfonso, Teresa B.
AU - Cohen, Daniela
AU - Dai, Haining
AU - Nery, Susana
AU - Stoica, Bogdan
AU - Slotkin, Jonathan
AU - Bregman, Barbara S.
AU - Fishell, Gord
AU - Corbin, Joshua G.
PY - 2006/11/8
Y1 - 2006/11/8
N2 - During embryogenesis, the lateral cortical stream (LCS) emerges from the corticostriatal border (CSB), the boundary between the developing cerebral cortex and striatum. The LCS is comprised of a mix of pallial- and subpallial-derived neural progenitor cells that migrate to the developing structures of the basal telencephalon, most notably the piriform cortex and amygdala. Using a combination of in vitro and in vivo approaches, we analyzed the timing, composition, migratory modes, origin, and requirement of the homeodomain-containing transcription factor Gsh2 (genomic screened homeobox 2) in the development of this prominent migratory stream. We reveal that Pax6 (paired box gene 6)-positive pallial-derived and Dlx2 (distal-less homeobox 2)-positive subpallial-derived subpopulations of LCS cells are generated in distinct temporal windows during embryogenesis. Furthermore, our data indicate the CSB border not only is comprised of separate populations of pallial- and subpallial-derived progenitors that contribute to the LCS but also a subpopulation of cells coexpressing Pax6 and Dlx2. Moreover, despite migrating along a route outlined by a cascade of radial glia, the Dlx2-positive population appears to migrate primarily in an apparent chain-like manner, with LCS migratory cells being generated locally at the CSB with little contribution from other subpallial structures such as the medial, lateral, or caudal ganglionic eminences. We further demonstrate that the generation of the LCS is dependent on the homeodomain-containing gene Gsh2, revealing a novel requirement for Gsh2 in telencephalic development.
AB - During embryogenesis, the lateral cortical stream (LCS) emerges from the corticostriatal border (CSB), the boundary between the developing cerebral cortex and striatum. The LCS is comprised of a mix of pallial- and subpallial-derived neural progenitor cells that migrate to the developing structures of the basal telencephalon, most notably the piriform cortex and amygdala. Using a combination of in vitro and in vivo approaches, we analyzed the timing, composition, migratory modes, origin, and requirement of the homeodomain-containing transcription factor Gsh2 (genomic screened homeobox 2) in the development of this prominent migratory stream. We reveal that Pax6 (paired box gene 6)-positive pallial-derived and Dlx2 (distal-less homeobox 2)-positive subpallial-derived subpopulations of LCS cells are generated in distinct temporal windows during embryogenesis. Furthermore, our data indicate the CSB border not only is comprised of separate populations of pallial- and subpallial-derived progenitors that contribute to the LCS but also a subpopulation of cells coexpressing Pax6 and Dlx2. Moreover, despite migrating along a route outlined by a cascade of radial glia, the Dlx2-positive population appears to migrate primarily in an apparent chain-like manner, with LCS migratory cells being generated locally at the CSB with little contribution from other subpallial structures such as the medial, lateral, or caudal ganglionic eminences. We further demonstrate that the generation of the LCS is dependent on the homeodomain-containing gene Gsh2, revealing a novel requirement for Gsh2 in telencephalic development.
KW - Amygdala
KW - Development
KW - Pallium
KW - Radial glia
KW - Specification
KW - Subpallium
UR - http://www.scopus.com/inward/record.url?scp=33751090335&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751090335&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.3092-06.2006
DO - 10.1523/JNEUROSCI.3092-06.2006
M3 - Article
C2 - 17093077
AN - SCOPUS:33751090335
SN - 0270-6474
VL - 26
SP - 11562
EP - 11574
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 45
ER -