TY - JOUR
T1 - Cellular binding of benzo(a)pyrene to DNA characterized by low temperature fluorescence
AU - Ivanovic, Vesna
AU - Geacintov, Nicholas E.
AU - Weinstein, I. Bernard
N1 - Funding Information:
The authors thank Dr. Ronald G. Harvey for supplying BP diol-epoxide (I) and (II) and BP 4,5-oxide. We are grateful to Drs. Steven H. Blobstein and Karen W. Jennette for preparing the --in vitro modified DNAs and to Mr. Douglas Eisenberg for preparing the hamster embryo cell cultures. This research was supported by NC1 Grant CA 02332 and NC1 Contract 3234 to I.B.W. and NC1 Grant CA 14980 to N.E.G.
PY - 1976/6/21
Y1 - 1976/6/21
N2 - A new approach has been developed to detect ultra low concentrations of benzo(a)pyrene products bound to nucleic acids in vivo. The binding to DNA of hamster embryo cell cultures was characterized by low temperature fluorescence spectroscopy. The method can detect less than one polycyclic hydrocarbon residue per 50,000 nucleotides. The fluorescence spectra indicate that the benzo(a)pyrene derivative bound to DNA has a pyrene-like chromophore and resembles that obtained when DNA is reacted in vitro with the 7,8-diol-9,10-oxide of benzo(a)pyrene. This confirms that metabolism of the 7,8,9,10 ring on benzo(a)pyrene precedes reaction with DNA. The method should be useful for detecting and characterizing the in vivo binding of other fluorescent carcinogens to nucleic acids.
AB - A new approach has been developed to detect ultra low concentrations of benzo(a)pyrene products bound to nucleic acids in vivo. The binding to DNA of hamster embryo cell cultures was characterized by low temperature fluorescence spectroscopy. The method can detect less than one polycyclic hydrocarbon residue per 50,000 nucleotides. The fluorescence spectra indicate that the benzo(a)pyrene derivative bound to DNA has a pyrene-like chromophore and resembles that obtained when DNA is reacted in vitro with the 7,8-diol-9,10-oxide of benzo(a)pyrene. This confirms that metabolism of the 7,8,9,10 ring on benzo(a)pyrene precedes reaction with DNA. The method should be useful for detecting and characterizing the in vivo binding of other fluorescent carcinogens to nucleic acids.
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U2 - 10.1016/0006-291X(76)91026-3
DO - 10.1016/0006-291X(76)91026-3
M3 - Article
C2 - 942439
AN - SCOPUS:0017175122
SN - 0006-291X
VL - 70
SP - 1172
EP - 1179
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -