Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

Ken Ichiro Kamei; Tetsuya Haruyama; Masayasu Mie; Yasuko Yanagida; Eiry Kobatake; Masuo Aizawa

doi:10.1023/A:1022339929026

Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

Ken Ichiro Kamei, Tetsuya Haruyama, Masayasu Mie, Yasuko Yanagida, Eiry Kobatake, Masuo Aizawa

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals, because some are known to induce NO synthase (iNOS) activity thereby increasing NO production. The macrophage-like cell line, RAW264.7, was cultured on the electrode coated with a polyion complex layer. The potent immune activating abilities of lipopolysaccharide could be verified by the cellular biosensing system: NO release from cells was detected within 600 ms by double potential step chronoamperometry.

Original language	English (US)
Pages (from-to)	321-325
Number of pages	5
Journal	Biotechnology Letters
Volume	25
Issue number	4
DOIs	https://doi.org/10.1023/A:1022339929026
State	Published - Feb 2003

Keywords

Cellular biosensing system
Inducible nitric oxide synthase
Lipopolysaccharide
Macrophage cell line
Nitric oxide
Polyion complex

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1023/A:1022339929026

Cite this

@article{6858881814be41258f8cce99ba24599f,

title = "Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade",

abstract = "A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals, because some are known to induce NO synthase (iNOS) activity thereby increasing NO production. The macrophage-like cell line, RAW264.7, was cultured on the electrode coated with a polyion complex layer. The potent immune activating abilities of lipopolysaccharide could be verified by the cellular biosensing system: NO release from cells was detected within 600 ms by double potential step chronoamperometry.",

keywords = "Cellular biosensing system, Inducible nitric oxide synthase, Lipopolysaccharide, Macrophage cell line, Nitric oxide, Polyion complex",

author = "Kamei, {Ken Ichiro} and Tetsuya Haruyama and Masayasu Mie and Yasuko Yanagida and Eiry Kobatake and Masuo Aizawa",

year = "2003",

month = feb,

doi = "10.1023/A:1022339929026",

language = "English (US)",

volume = "25",

pages = "321--325",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "4",

}

TY - JOUR

T1 - Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

AU - Kamei, Ken Ichiro

AU - Haruyama, Tetsuya

AU - Mie, Masayasu

AU - Yanagida, Yasuko

AU - Kobatake, Eiry

AU - Aizawa, Masuo

PY - 2003/2

Y1 - 2003/2

N2 - A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals, because some are known to induce NO synthase (iNOS) activity thereby increasing NO production. The macrophage-like cell line, RAW264.7, was cultured on the electrode coated with a polyion complex layer. The potent immune activating abilities of lipopolysaccharide could be verified by the cellular biosensing system: NO release from cells was detected within 600 ms by double potential step chronoamperometry.

AB - A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals, because some are known to induce NO synthase (iNOS) activity thereby increasing NO production. The macrophage-like cell line, RAW264.7, was cultured on the electrode coated with a polyion complex layer. The potent immune activating abilities of lipopolysaccharide could be verified by the cellular biosensing system: NO release from cells was detected within 600 ms by double potential step chronoamperometry.

KW - Cellular biosensing system

KW - Inducible nitric oxide synthase

KW - Lipopolysaccharide

KW - Macrophage cell line

KW - Nitric oxide

KW - Polyion complex

UR - http://www.scopus.com/inward/record.url?scp=0037298068&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037298068&partnerID=8YFLogxK

U2 - 10.1023/A:1022339929026

DO - 10.1023/A:1022339929026

M3 - Article

C2 - 12882545

AN - SCOPUS:0037298068

SN - 0141-5492

VL - 25

SP - 321

EP - 325

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 4

ER -

Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this