Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates

Jason M. Belitsky; Stephanie J. Leslie; Paramjit S. Arora; Terry A. Beerman; Peter B. Dervan

doi:10.1016/S0968-0896(02)00204-3

Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates

Jason M. Belitsky, Stephanie J. Leslie, Paramjit S. Arora, Terry A. Beerman, Peter B. Dervan

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide dye conjugates in a variety of living ells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.

Original language	English (US)
Pages (from-to)	3313-3318
Number of pages	6
Journal	Bioorganic and Medicinal Chemistry
Volume	10
Issue number	10
DOIs	https://doi.org/10.1016/S0968-0896(02)00204-3
State	Published - 2002

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/S0968-0896(02)00204-3

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title = "Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates",

abstract = "The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide dye conjugates in a variety of living ells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.",

author = "Belitsky, {Jason M.} and Leslie, {Stephanie J.} and Arora, {Paramjit S.} and Beerman, {Terry A.} and Dervan, {Peter B.}",

note = "Funding Information: We are grateful to Scott Fraser and Hayong Lim for use of the Caltech Biological Imaging Facility and assistance in confocal microscopy. Special thanks to Inder Nangiana of the Caltech Protein Expression Facility for help with cell culture. We are indebted to Aseem Z. Ansari, Joel Pomerantz, Joel Gottesfeld, and Carl Parker for providing cells and helpful suggestions. We are grateful to the National Institutes of Health (GM 51747) for research support, the Ralph M. Parsons Foundation for a predoctoral fellowship to J.M.B., and the American Cancer Society for a post-doctoral fellowship to P.S.A.",

year = "2002",

doi = "10.1016/S0968-0896(02)00204-3",

language = "English (US)",

volume = "10",

pages = "3313--3318",

journal = "Bioorganic and Medicinal Chemistry",

issn = "0968-0896",

publisher = "Elsevier Limited",

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T1 - Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates

AU - Belitsky, Jason M.

AU - Leslie, Stephanie J.

AU - Arora, Paramjit S.

AU - Beerman, Terry A.

AU - Dervan, Peter B.

N1 - Funding Information: We are grateful to Scott Fraser and Hayong Lim for use of the Caltech Biological Imaging Facility and assistance in confocal microscopy. Special thanks to Inder Nangiana of the Caltech Protein Expression Facility for help with cell culture. We are indebted to Aseem Z. Ansari, Joel Pomerantz, Joel Gottesfeld, and Carl Parker for providing cells and helpful suggestions. We are grateful to the National Institutes of Health (GM 51747) for research support, the Ralph M. Parsons Foundation for a predoctoral fellowship to J.M.B., and the American Cancer Society for a post-doctoral fellowship to P.S.A.

PY - 2002

Y1 - 2002

N2 - The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide dye conjugates in a variety of living ells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.

AB - The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide dye conjugates in a variety of living ells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.

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ER -

Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates

Abstract

ASJC Scopus subject areas

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