Ceramide triggers intracellular calcium release via the IP3 receptor in Xenopus laevis oocytes

Evgeny Kobrinsky, Andrew L. Spielman, Sophia Rosenzweig, Andrew R. Marks

Research output: Contribution to journalArticle

Abstract

Ceramide, a product of sphingomyelin turnover, is a lipid second messenger that mediates diverse signaling pathways, including those leading to cell cycle arrest and differentiation. The mechanism(s) by which ceramide signals downstream events have not been fully elucidated. Here we show that, in Xenopus laevis oocytes, ceramide-induced maturation is associated with the release of intracellular calcium stores. Ceramide caused a dose-dependent elevation in the second messenger inositol 1,4,5-trisphosphate (IP3) via activation of G(q/11)α and phospholipase C-βX. Elevation of IP3, in turn, activated the IP3 receptor calcium release channel on the endoplasmic reticulum, resulting in a rise in cytoplasmic calcium. Thus our study demonstrates that cross talk between the ceramide and phosphoinositide signaling pathways modulates intracellular calcium homeostasis.

Original languageEnglish (US)
Pages (from-to)C665-C672
JournalAmerican Journal of Physiology - Cell Physiology
Volume277
Issue number4 46-4
DOIs
StatePublished - 1999

Keywords

  • Calcium signaling
  • Meiotic maturation
  • Phospholipase C

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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