TY - JOUR
T1 - Changes in whole body lipid composition in a murine model of insulin-dependent diabetes mellitus
AU - Rabinowitz, Joseph L.
AU - Craig, Ronald G.
N1 - Funding Information:
From the Veterans Administration Medical Center, Philadelphia and the Department of Biochemistry. University of Pennsylvania School of Dental Medicine. Philadelphia. This study was supported by award #I86153 from the Juvenile Diabetes Foundation International to R. Craig. Address reprint requests to Joseph L. Rabinowitz. PhD, Veterans Administration Medical Center, University and Woodland Aves, Philadelphia, PA 19104. 0 1989 by Grune & Stratton, Inc. 0026-0495/89/3808-0014$03.00/O
PY - 1989/8
Y1 - 1989/8
N2 - To assess the effect of experimentally induced insulin-dependent diabetes mellitus (IDDM) on total body lipid composition, homogenates of neonatal (0-day) and 6-day Sprague-Dawley rat pups treated on day 0 with 65 mg/kg body weight of streptozotocin (STZ) or citrate buffer alone were compared using thin-layer and gas-liquid chromatographic techniques. STZ-treated littermates in a parallel study were markedly hyperglycemic, hypoinsulinemic and attained only 50% of the gain in weight of citrate-treated controls. Although both groups were similar in protein to body weight ratios, STZ-treated pups exhibited 60% of the total lipid content of citrate-treated littermates when compared by weight. The decrease in total lipid content in the STZ-treated group resulted specifically from decreased neutral and not phospholipid content, although a small increase in phosphatidic acid and sphigomyelin was observed in this group. The changes in relative whole body lipids with short-term, high-dose STZ parallel those reported in human IDDM.
AB - To assess the effect of experimentally induced insulin-dependent diabetes mellitus (IDDM) on total body lipid composition, homogenates of neonatal (0-day) and 6-day Sprague-Dawley rat pups treated on day 0 with 65 mg/kg body weight of streptozotocin (STZ) or citrate buffer alone were compared using thin-layer and gas-liquid chromatographic techniques. STZ-treated littermates in a parallel study were markedly hyperglycemic, hypoinsulinemic and attained only 50% of the gain in weight of citrate-treated controls. Although both groups were similar in protein to body weight ratios, STZ-treated pups exhibited 60% of the total lipid content of citrate-treated littermates when compared by weight. The decrease in total lipid content in the STZ-treated group resulted specifically from decreased neutral and not phospholipid content, although a small increase in phosphatidic acid and sphigomyelin was observed in this group. The changes in relative whole body lipids with short-term, high-dose STZ parallel those reported in human IDDM.
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U2 - 10.1016/0026-0495(89)90066-8
DO - 10.1016/0026-0495(89)90066-8
M3 - Article
C2 - 2761414
AN - SCOPUS:0024318363
SN - 0026-0495
VL - 38
SP - 777
EP - 780
JO - Metabolism
JF - Metabolism
IS - 8
ER -