Channels in the mitochondrial outer membrane: Evidence from patch clamp studies

Henry Tedeschi, Kathleen Walsh Kinnally

Research output: Contribution to journalArticlepeer-review

Abstract

Patch clamp techniques were applied to outer mitochondrial membranes of giant mitochondria from mice kept on a cuprizone diet or to vesicles produced by fusing membranes derived from the outer membrane of Neurospora mitochondria. In the negative range of potentials the conductances decreased with increases in the magnitude of voltage, suggesting the closing of channels. Experiments in which mitochondria were treated with the polyanion polymethacrylate maleate styrene (1:2:3) or succinic anhydride suggest that the channels correspond to VDAC. Although sometimes conductance also decreased with increasing potential over a narrow range of positive potentials, more commonly the conductances increased. Although this phenomenon may represent a detachment of the patch, the changes in conductance are reversible, suggesting that they correspond to the formation or the opening of channels.

Original languageEnglish (US)
Pages (from-to)321-327
Number of pages7
JournalJournal of Bioenergetics and Biomembranes
Volume19
Issue number4
DOIs
StatePublished - Aug 1987

Keywords

  • Mitochondrial outer membrane
  • channels
  • mitochondrial channels

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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