Characterisation of cytoplasmic DNA complementary to non-retroviral RNA viruses in human cells

Akira Shimizu, Yoko Nakatani, Takako Nakamura, Atsushi Jinno-Oue, Osamu Ishikawa, Jef D. Boeke, Yasuhiro Takeuchi, Hiroo Hoshino

    Research output: Contribution to journalArticle

    Abstract

    The synthesis and subsequent genomic integration of DNA that is complementary to the genomes of non-retroviral RNA viruses are rarely observed. However, upon infection of various human cell lines and primary fibroblasts with the vesicular stomatitis virus (VSV), we detected DNA complementary to the VSV RNA. The VSV DNA was detected in the cytoplasm as single-stranded DNA fully complementary to the viral mRNA from the poly(A) region to the 7-methyl guanosine cap. The formation of this DNA was cell-dependent. Experimentally, we found that the transduction of cells that do not produce VSV DNA with the long interspersed nuclear element 1 and their infection with VSV could lead to the formation of VSV DNA. Viral DNA complementary to other RNA viruses was also detected in the respective infected human cells. Thus, the genetic information of the non-retroviral RNA virus genome can flow into the DNA of mammalian cells expressing LINE-1-like elements.

    Original languageEnglish (US)
    Article number5074
    JournalScientific reports
    Volume4
    DOIs
    StatePublished - May 29 2014

    ASJC Scopus subject areas

    • General

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    Shimizu, A., Nakatani, Y., Nakamura, T., Jinno-Oue, A., Ishikawa, O., Boeke, J. D., Takeuchi, Y., & Hoshino, H. (2014). Characterisation of cytoplasmic DNA complementary to non-retroviral RNA viruses in human cells. Scientific reports, 4, [5074]. https://doi.org/10.1038/srep05074