Characterization of a lymphokine produced by human T cells which inhibits collagen synthesis

Joel Rosenbloom; William McArthur; Daniel Malamud; Sergio Jimenez

doi:10.1016/0008-8749(83)90226-5

Characterization of a lymphokine produced by human T cells which inhibits collagen synthesis

Joel Rosenbloom, William McArthur, Daniel Malamud, Sergio Jimenez

Research output: Contribution to journal › Article › peer-review

Abstract

Human lymphocytes, isolated from peripheral blood, were cultured for 48 hr in a defined medium containing 10 mg/ml bovine serum albumin and phytohemagglutinin. A lymphokine which inhibits collagen synthesis by cultured human dermal fibroblasts was purified from the lymphocyte incubation medium by successive steps of ammonium sulfate precipitation, gel filtration chromatography, and isoelectric focusing. Good recovery of this collagen synthesis inhibitory factor (CSIF) was obtained and a factor with an approximate molecular weight of 55,000 and a pI of 6.2 was isolated. The purification of the factor should permit further studies on its mechanism of action.

Original language	English (US)
Pages (from-to)	192-198
Number of pages	7
Journal	Cellular Immunology
Volume	81
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(83)90226-5
State	Published - Oct 1 1983

ASJC Scopus subject areas

Immunology

Access to Document

10.1016/0008-8749(83)90226-5

Cite this

@article{49804cef8bb84c5cb6b3aa50417cd7ea,

title = "Characterization of a lymphokine produced by human T cells which inhibits collagen synthesis",

abstract = "Human lymphocytes, isolated from peripheral blood, were cultured for 48 hr in a defined medium containing 10 mg/ml bovine serum albumin and phytohemagglutinin. A lymphokine which inhibits collagen synthesis by cultured human dermal fibroblasts was purified from the lymphocyte incubation medium by successive steps of ammonium sulfate precipitation, gel filtration chromatography, and isoelectric focusing. Good recovery of this collagen synthesis inhibitory factor (CSIF) was obtained and a factor with an approximate molecular weight of 55,000 and a pI of 6.2 was isolated. The purification of the factor should permit further studies on its mechanism of action.",

author = "Joel Rosenbloom and William McArthur and Daniel Malamud and Sergio Jimenez",

note = "Funding Information: This research was supported by U.S. Public Health Service Grants DE-02623, AM-02863, and AM-19616 from the National Institutes of Health. The authors would like to thank Ms. Mary K. Dixon for superior technical assistance.",

year = "1983",

month = oct,

day = "1",

doi = "10.1016/0008-8749(83)90226-5",

language = "English (US)",

volume = "81",

pages = "192--198",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Characterization of a lymphokine produced by human T cells which inhibits collagen synthesis

AU - Rosenbloom, Joel

AU - McArthur, William

AU - Malamud, Daniel

AU - Jimenez, Sergio

N1 - Funding Information: This research was supported by U.S. Public Health Service Grants DE-02623, AM-02863, and AM-19616 from the National Institutes of Health. The authors would like to thank Ms. Mary K. Dixon for superior technical assistance.

PY - 1983/10/1

Y1 - 1983/10/1

N2 - Human lymphocytes, isolated from peripheral blood, were cultured for 48 hr in a defined medium containing 10 mg/ml bovine serum albumin and phytohemagglutinin. A lymphokine which inhibits collagen synthesis by cultured human dermal fibroblasts was purified from the lymphocyte incubation medium by successive steps of ammonium sulfate precipitation, gel filtration chromatography, and isoelectric focusing. Good recovery of this collagen synthesis inhibitory factor (CSIF) was obtained and a factor with an approximate molecular weight of 55,000 and a pI of 6.2 was isolated. The purification of the factor should permit further studies on its mechanism of action.

AB - Human lymphocytes, isolated from peripheral blood, were cultured for 48 hr in a defined medium containing 10 mg/ml bovine serum albumin and phytohemagglutinin. A lymphokine which inhibits collagen synthesis by cultured human dermal fibroblasts was purified from the lymphocyte incubation medium by successive steps of ammonium sulfate precipitation, gel filtration chromatography, and isoelectric focusing. Good recovery of this collagen synthesis inhibitory factor (CSIF) was obtained and a factor with an approximate molecular weight of 55,000 and a pI of 6.2 was isolated. The purification of the factor should permit further studies on its mechanism of action.

UR - http://www.scopus.com/inward/record.url?scp=0020553136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020553136&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(83)90226-5

DO - 10.1016/0008-8749(83)90226-5

M3 - Article

C2 - 6604586

AN - SCOPUS:0020553136

SN - 0008-8749

VL - 81

SP - 192

EP - 198

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Characterization of a lymphokine produced by human T cells which inhibits collagen synthesis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this