Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin

S. M. Forrest, K. W. Ng, D. M. Findlay, V. P. Michelangeli, S. A. Livesey, N. C. Partridge, J. D. Zajac, T. J. Martin

Research output: Contribution to journalArticle

Abstract

The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages. Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06). Both subclones responded in an identical manner to PTH, which stimulated adenylate cyclase and activated both isoenzyme I and isoenzyme II of cyclic AMP-dependent protein kinase. In contrast, only UMR 106-06 cells responded to calcitonin. At 3×10-8M SCT, there was a sevenfold stimulation of adenylate cyclase, 84% activation of isoenzyme I, and 44% activation of isoenzyme II. The activation profiles of the isoenzymes to PTH and SCT in UMR 106-06 were similar. Furthermore, their response to SCT correlates with the presence of specific, saturable binding of125I-labeled SCT. Binding parameters indicate apparent Kd=0.8 nM and 6,000 receptors/cell. These data point to a significant phenotypic change having taken place in this clonal cell line with prolonged maintenance in culture, with the emergence of a calcitonin receptor linked to adenylate cyclase and protein kinase activation.

Original languageEnglish (US)
Pages (from-to)51-56
Number of pages6
JournalCalcified Tissue International
Volume37
Issue number1
DOIs
StatePublished - Jan 1985

Keywords

  • Calcitonin
  • Cyclic AMP
  • Differentiation
  • Osteoblast
  • Parathyroid hormone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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    Forrest, S. M., Ng, K. W., Findlay, D. M., Michelangeli, V. P., Livesey, S. A., Partridge, N. C., Zajac, J. D., & Martin, T. J. (1985). Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin. Calcified Tissue International, 37(1), 51-56. https://doi.org/10.1007/BF02557679