Characterization of bovine patellar cartilage by NMR

Wen Ling, Ravinder R. Regatte, Mark E. Schweitzer, Alexej Jerschow

Research output: Contribution to journalArticle

Abstract

Metabolic and structural changes in cartilage tissue are thought to be at the root of degenerative joint disease. We identify here the NMR resonances in bovine patellar cartilage tissue by static and high-resolution magic angle spinning (HRMAS) NMR spectroscopy, 1H-13C heteronuclear single-quantum correlation (HSQC) spectroscopy, total correlation spectroscopy (TOCSY), and saturation transfer experiments. Some differences between the patellar cartilage samples studied here and earlier nasal cartilage and intervertebrate disc studies were found. In addition, we show assignments downfield of the water signal, which also includes the assignment of amide and hydroxy protons on the basis of their exchangeability with water. These results will allow an identification of spectroscopic markers of cartilage degradation using techniques such as chemical exchange saturation transfer imaging.

Original languageEnglish (US)
Pages (from-to)289-295
Number of pages7
JournalNMR in Biomedicine
Volume21
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • Cartilage
  • Chemical exchange
  • Chemical exchange saturation transfer (CEST)
  • MRI
  • Osteoarthritis
  • Proteoglycan assessment

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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