Characterization of Nkx6-2-derived neocortical interneuron lineages

Vitor H. Sousa, Goichi Miyoshi, Jens Hjerling-Leffler, Theofanis Karayannis, Gord Fishell

Research output: Contribution to journalArticle

Abstract

Ventral telencephalic progenitors expressing the homeodomain transcription factor Nkx6-2 have been shown to give rise to a multitude of cortical interneuron subtypes usually associated with origin in either the medial ganglionic eminence or the caudal ganglionic eminence. The function of Nkx6-2 in directing the fate of those progenitors has, however, not been thoroughly analyzed. We used a combination of genetic inducible fate mapping and in vivo lossof-function to analyze the requirement of Nkx6-2 in determining the fate of cortical interneurons. We have found that interneuron subtypes are born with a characteristic temporal pattern. Furthermore, we extend the characterization of interneurons from the Nkx6-2 lineage through the application of electrophysiological methods. Analysis of these populations in Nkx6-2 null mice suggests that there is a small and partially penetrant loss of delayed non-fast spiking somatostatin/calretinin double positive cortical interneurons in the absence of Nkx6-2 gene function.

Original languageEnglish (US)
Pages (from-to)i1-i10
JournalCerebral Cortex
Volume19
Issue numberSUPPL. 1
DOIs
StatePublished - Jul 2009

Keywords

  • Genetic fate mapping
  • Interganglionic sulcus
  • Loss of function
  • Mouse genetics
  • Whole-cell physiology

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

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    Sousa, V. H., Miyoshi, G., Hjerling-Leffler, J., Karayannis, T., & Fishell, G. (2009). Characterization of Nkx6-2-derived neocortical interneuron lineages. Cerebral Cortex, 19(SUPPL. 1), i1-i10. https://doi.org/10.1093/cercor/bhp038