Characterization of specific binding sites labeled by [3H]LSD in coronal sections of paraformaldehyde-fixed rat brain

Sheryl G. Beck, Efrain C. Azmitia

Research output: Contribution to journalArticle

Abstract

Specific, high-affinity binding of [3H]lysergic acid diethylamide (LSD) in coronal sections of paraformaldehyde-fixed rat brain is described. Intracardiac perfusion of paraformaldehyde selectively altered 40% of the total binding sites normally labeled by [3H]LSD in unfixed sections. Competition by unlabeled LSD and serotonin (5-HT) was not altered by the fixation procedure. Competition by spiperone, however, revealed that the fixation procedure preferentially altered sites for which spiperone has high affinity. This technique should facilitate the combination of neuroanatomical techniques such as radioautography and immunocytochemistry.

Original languageEnglish (US)
Pages (from-to)369-373
Number of pages5
JournalBrain Research
Volume345
Issue number2
DOIs
StatePublished - Oct 21 1985

Keywords

  • 5-hydroxytryptamine (5-HT)
  • [H]lysergic acid diethylamide ([H]LSD)
  • binding site
  • paraformaldehyde fixation
  • spiperone

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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