TY - JOUR
T1 - Characterizing Antibody Responses to Plasmodium vivax and Plasmodium falciparum Antigens in India Using Genome-Scale Protein Microarrays
AU - Uplekar, Swapna
AU - Rao, Pavitra Nagesh
AU - Ramanathapuram, Lalitha
AU - Awasthi, Vikky
AU - Verma, Kalpana
AU - Sutton, Patrick
AU - Ali, Syed Zeeshan
AU - Patel, Ankita
AU - Sri Lakshmi Priya, G.
AU - Ravishankaran, Sangamithra
AU - Desai, Nisha
AU - Tandel, Nikunj
AU - Choubey, Sandhya
AU - Barla, Punam
AU - Kanagaraj, Deena
AU - Eapen, Alex
AU - Pradhan, Khageswar
AU - Singh, Ranvir
AU - Jain, Aarti
AU - Felgner, Philip L.
AU - Davies, D. Huw
AU - Carlton, Jane M.
AU - Das, Jyoti
N1 - Publisher Copyright:
© 2017 Uplekar et al.
PY - 2017/1/24
Y1 - 2017/1/24
N2 - Understanding naturally acquired immune responses to Plasmodium in India is key to improving malaria surveillance and diagnostic tools. Here we describe serological profiling of immune responses at three sites in India by probing protein microarrays consisting of 515 Plasmodium vivax and 500 Plasmodium falciparum proteins with 353 plasma samples. A total of 236 malaria-positive (symptomatic and asymptomatic) plasma samples and 117 malaria-negative samples were collected at three field sites in Raurkela, Nadiad, and Chennai. Indian samples showed significant seroreactivity to 265 P. vivax and 373 P. falciparum antigens, but overall seroreactivity to P. vivax antigens was lower compared to P. falciparum antigens. We identified the most immunogenic antigens of both Plasmodium species that were recognized at all three sites in India, as well as P. falciparum antigens that were associated with asymptomatic malaria. This is the first genome-scale analysis of serological responses to the two major species of malaria parasite in India. The range of immune responses characterized in different endemic settings argues for targeted surveillance approaches tailored to the diverse epidemiology of malaria across the world.
AB - Understanding naturally acquired immune responses to Plasmodium in India is key to improving malaria surveillance and diagnostic tools. Here we describe serological profiling of immune responses at three sites in India by probing protein microarrays consisting of 515 Plasmodium vivax and 500 Plasmodium falciparum proteins with 353 plasma samples. A total of 236 malaria-positive (symptomatic and asymptomatic) plasma samples and 117 malaria-negative samples were collected at three field sites in Raurkela, Nadiad, and Chennai. Indian samples showed significant seroreactivity to 265 P. vivax and 373 P. falciparum antigens, but overall seroreactivity to P. vivax antigens was lower compared to P. falciparum antigens. We identified the most immunogenic antigens of both Plasmodium species that were recognized at all three sites in India, as well as P. falciparum antigens that were associated with asymptomatic malaria. This is the first genome-scale analysis of serological responses to the two major species of malaria parasite in India. The range of immune responses characterized in different endemic settings argues for targeted surveillance approaches tailored to the diverse epidemiology of malaria across the world.
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U2 - 10.1371/journal.pntd.0005323
DO - 10.1371/journal.pntd.0005323
M3 - Article
C2 - 28118367
AN - SCOPUS:85012912958
SN - 1935-2727
VL - 11
JO - PLoS neglected tropical diseases
JF - PLoS neglected tropical diseases
IS - 1
M1 - e0005323
ER -