Chemically-defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions.

L. Liu, Qinghua Yuan, Jian Shi, Xin Li, Dongju Jung, L. Wang, Kaori Yamauchi, Norio Nakatsuji, Ken ichiro Kamei, Yong Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Embryonic stem cells (ESCs) are useful resources for drug discovery, developmental biology and disease studies. Cellular microenvironmental cues play critical roles in regulating ESC functions, but it is challenging to control them with synthetic components. Nanofibers hold a potential to create artificial cellular cues for controlling cell adhesion and cell-cell interactions. Mouse ESC (mESC) were cultured on electrospun nanofibers made from polymethylglutarimide (PMGI), which is a synthetic thermoplastic polymer stable under culture conditions. Both topology and the density of PMGI nanofibers were key factors. mESCs on nanofibers had a growth rate comparable to those cultured conventionally and retained their pluripotency. Furthermore, self-renewed ESCs differentiated into all three germ layers thereby providing a reliable way to expand mESCs without feeder cells.

Original languageEnglish (US)
Pages (from-to)1951-1957
Number of pages7
JournalBiotechnology Letters
Volume34
Issue number10
DOIs
StatePublished - Oct 2012

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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