Cholecalcin (a 9‐kDa cholecalciferol‐induced calcium‐binding protein) messenger RNA: Distribution and induction by calcitriol in the rat digestive tract

In view of the possible physiological importance of the 9‐kDa cholecalcin (a 9000‐M_r cholecalciferol‐induced calcium‐binding protein) in the intestinal transport of calcium in mammals, the gene expression of this protein has been analysed. Its regulation in the digestive tract of the growing rat by calcitriol (1,25‐dihydroxycholecalciferol) was studied using a specific cloned [³²P]cDNA to 9‐kDa cholecalcin. Northern hybridisation studies show that the cDNA sequence hybridises to a single 500–600‐nucleotide species throughout the digestive tract and therefore demonstrate identical 9‐kDa‐cholecalin mRNA processing in the whole of the intestine and caecum. The highest concentrations of cholecalcin mRNA occur in the duodenum, proximal jejunum and caecum. The observed differences in 9‐kDa‐cholecalcin mRNA levels correlate well with both the in vivo variations in cholecalcin itself and with the known intestinal sites of calcium absorption. The whole intestine is able to respond to exogenous calcitriol but the response of the distal intestine and caecum, as measured by the increase in cholecalcin mRNA and corresponding protein, was proportionally higher than in the duodenum. The rapid production of fully functional cholecalcin mRNA, which was detectable as early as 1 h after a single dose of calcitriol to vitamin‐D‐deficient rats, provides convincing evidence that calcitriol increases 9‐kDa cholecalcin production by increasing cholecalcin gene expression at the transcriptional level.