TY - GEN
T1 - Chondrocyte maturation on biphasic calcium phosphate scaffold
T2 - Transactions - 7th World Biomaterials Congress
AU - Teixeira, C. C.
AU - LeGeros, R. Z.
AU - Karkia, C.
AU - Nemelivsky, Y.
PY - 2004
Y1 - 2004
N2 - A method of growing chondrocytes in a well characterized biphasic calcium phosphate (BCP) scaffold was studied. Chondrocytes were grown in culture continuously for 3 weeks, and fed every day with Dulbecco's modified Eagle medium (GIBCO) containing 10% Nu serum, 2mM L-glutamine, and 50 μg/ml ascorbic acid. Chondrocytes grown on BCP scaffold assumed an elongated morphology within an intricate net of collagenous protein, while cells grown on tissue culture plate surfaces maintained a polygon and flat morphology. The results show that a calcium phosphate scaffold can be used as a template for chondrocyte proliferation and maturation.
AB - A method of growing chondrocytes in a well characterized biphasic calcium phosphate (BCP) scaffold was studied. Chondrocytes were grown in culture continuously for 3 weeks, and fed every day with Dulbecco's modified Eagle medium (GIBCO) containing 10% Nu serum, 2mM L-glutamine, and 50 μg/ml ascorbic acid. Chondrocytes grown on BCP scaffold assumed an elongated morphology within an intricate net of collagenous protein, while cells grown on tissue culture plate surfaces maintained a polygon and flat morphology. The results show that a calcium phosphate scaffold can be used as a template for chondrocyte proliferation and maturation.
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M3 - Conference contribution
AN - SCOPUS:13844303625
SN - 1877040193
SN - 9781877040191
T3 - Transactions - 7th World Biomaterials Congress
BT - Transactions - 7th World Biomaterials Congress
Y2 - 17 May 2004 through 21 May 2004
ER -