TY - JOUR
T1 - Circulating microRNAs are associated with variability in fasting blood glucose over 12-months and target pathways related to type 2 diabetes
T2 - A pilot study
AU - Flowers, Elena
AU - Allen, Isabel E.
AU - Kanaya, Alka M.
AU - Aouizerat, Bradley E.
N1 - Funding Information:
Molecular data collection was performed by the Helen Diller Family Comprehensive Cancer Center Laboratory for Cell Analysis Shared Resource Facility (P30CA082103). The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by Dr Flowers who was supported by the National Center for Advancing Translational Sciences of the National Institutes of Health grant number KL2TR000143 and the Hellman Family Foundation. The PRYSMS study was supported by the National Center for Complementary and Alternative Medicine of the National Institutes of Health grant number R01AT004569. Molecular data collection from the PRYSMS study was supported by the National Institute of Diabetes, Digestive, and Kidney Disease of the National Institutes of Health grant number R21DK117346. Dr Kanaya is supported by National Heart Lung, and Blood Institute of the National Institutes of Health grant number 2K24HL112827.
Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by Dr Flowers who was supported by the National Center for Advancing Translational Sciences of the National Institutes of Health grant number KL2TR000143 and the Hellman Family Foundation. The PRYSMS study was supported by the National Center for Complementary and Alternative Medicine of the National Institutes of Health grant number R01AT004569. Molecular data collection from the PRYSMS study was supported by the National Institute of Diabetes, Digestive, and Kidney Disease of the National Institutes of Health grant number R21DK117346. Dr Kanaya is supported by National Heart Lung, and Blood Institute of the National Institutes of Health grant number 2K24HL112827.
Publisher Copyright:
© The Author(s) 2021.
PY - 2021/11/26
Y1 - 2021/11/26
N2 - Introduction: MicroRNAs (miRs) may be important regulators of risk for type 2 diabetes (T2D). Circulating miRs may provide information about which individuals are at risk for T2D. The purpose of this study was to assess longitudinal associations between circulating miR expression and variability in fasting blood glucose (FBG) and to identify miR-targeted genes and biological pathways. Methods: Variability in FBG was estimated using standard deviation from participants (n = 20) in a previously completed yoga trial. Expression of 402 miRs was measured using hydrogel particle lithography. MirTarBase was used to identify mRNAs, and miRPathDB was used to identify pathways targeted by differentially expressed miRs. Results: Six circulating miRs (miR-192, miR-197, miR-206, miR-424, miR-486, and miR-93) were associated with variability in FBG and targeted 143 genes and 23 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Six mRNAs (AKT1, CCND1, ESR1, FASN, SMAD7, and VEGFA) were targeted by at least two miRs and four of those were located in miR-targeted KEGG pathways. Conclusions: Circulating miRs are associated with variability in FBG in individuals at risk for T2D. Further studies are needed to determine whether miRs may be prodromal biomarkers that can identify which individuals are at greatest risk to progress to T2D and which biological pathways underlie this risk.
AB - Introduction: MicroRNAs (miRs) may be important regulators of risk for type 2 diabetes (T2D). Circulating miRs may provide information about which individuals are at risk for T2D. The purpose of this study was to assess longitudinal associations between circulating miR expression and variability in fasting blood glucose (FBG) and to identify miR-targeted genes and biological pathways. Methods: Variability in FBG was estimated using standard deviation from participants (n = 20) in a previously completed yoga trial. Expression of 402 miRs was measured using hydrogel particle lithography. MirTarBase was used to identify mRNAs, and miRPathDB was used to identify pathways targeted by differentially expressed miRs. Results: Six circulating miRs (miR-192, miR-197, miR-206, miR-424, miR-486, and miR-93) were associated with variability in FBG and targeted 143 genes and 23 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Six mRNAs (AKT1, CCND1, ESR1, FASN, SMAD7, and VEGFA) were targeted by at least two miRs and four of those were located in miR-targeted KEGG pathways. Conclusions: Circulating miRs are associated with variability in FBG in individuals at risk for T2D. Further studies are needed to determine whether miRs may be prodromal biomarkers that can identify which individuals are at greatest risk to progress to T2D and which biological pathways underlie this risk.
KW - biomarker
KW - diabetes
KW - fasting blood glucose
KW - microRNA
UR - http://www.scopus.com/inward/record.url?scp=85121618826&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85121618826&partnerID=8YFLogxK
U2 - 10.1177/14791641211055837
DO - 10.1177/14791641211055837
M3 - Article
C2 - 34846185
AN - SCOPUS:85121618826
SN - 1479-1641
VL - 18
JO - Diabetes and Vascular Disease Research
JF - Diabetes and Vascular Disease Research
IS - 6
ER -