Clonal analyses and gene profiling identify genetic biomarkers of the thermogenic potential of human brown and white preadipocytes

Ruidan Xue, Matthew D. Lynes, Jonathan M. Dreyfuss, Farnaz Shamsi, Tim J. Schulz, Hongbin Zhang, Tian Lian Huang, Kristy L. Townsend, Yiming Li, Hirokazu Takahashi, Lauren S. Weiner, Andrew P. White, Maureen S. Lynes, Lee L. Rubin, Laurie J. Goodyear, Aaron M. Cypess, Yu Hua Tseng

Research output: Contribution to journalArticlepeer-review

Abstract

Targeting brown adipose tissue (BAT) content or activity has therapeutic potential for treating obesity and the metabolic syndrome by increasing energy expenditure. However, both inter- and intra-individual differences contribute to heterogeneity in human BAT and potentially to differential thermogenic capacity in human populations. Here we generated clones of brown and white preadipocytes from human neck fat and characterized their adipogenic and thermogenic differentiation. We combined an uncoupling protein 1 (UCP1) reporter system and expression profiling to define novel sets of gene signatures in human preadipocytes that could predict the thermogenic potential of the cells once they were maturated. Knocking out the positive UCP1 regulators, PREX1 and EDNRB, in brown preadipocytes using CRISPR-Cas9 markedly abolished the high level of UCP1 in brown adipocytes differentiated from the preadipocytes. Finally, we were able to prospectively isolate adipose progenitors with great thermogenic potential using the cell surface marker CD29. These data provide new insights into the cellular heterogeneity in human fat and offer potential biomarkers for identifying thermogenically competent preadipocytes.

Original languageEnglish (US)
Pages (from-to)760-768
Number of pages9
JournalNature Medicine
Volume21
Issue number7
DOIs
StatePublished - Jul 9 2015

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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