Clonogenic Analysis Reveals Reserve Stem Cells in Postnatal Mammals. II. Pluripotent Epiblastic-Like Stem Cells

Henry E. Young; Cecile Duplaa; Michael J. Yost; Nicholas L. Henson; Julie A. Floyd; Kristina Detmer; Angela J. Thompson; Steven W. Powell; T. Clark Gamblin; Kirk Kizziah; Benjamin J. Holland; Angel Boev; J. M. Van De Water; Dan C. Godbee; Stephanie Jackson; Marylen Rimando; Chad R. Edwards; Eveline Wu; Chris Cawley; Pamela D. Edwards; Anna Macgregor; Ryan Bozof; T. Michele Thompson; George J. Petro; Heather M. Shelton; Beth L. McCampbell; Jared C. Mills; Frederick L. Flynt; Timothy A. Steele; Marianne Kearney; Amy Kirincich-Greathead; Wade Hardy; Paul R. Young; Aman V. Amin; R. Steve Williams; Miranda M. Horton; Shaun McGuinn; Kristina C. Hawkins; Kurt Ericson; Louis Terracio; Catherine Moreau; Douglas Hixson; Brian W. Tobin; John Hudson; Frank P. Bowyer; Asa C. Black

doi:10.1002/ar.a.20000

Clonogenic Analysis Reveals Reserve Stem Cells in Postnatal Mammals. II. Pluripotent Epiblastic-Like Stem Cells

Henry E. Young, Cecile Duplaa, Michael J. Yost, Nicholas L. Henson, Julie A. Floyd, Kristina Detmer, Angela J. Thompson, Steven W. Powell, T. Clark Gamblin, Kirk Kizziah, Benjamin J. Holland, Angel Boev, J. M. Van De Water, Dan C. Godbee, Stephanie Jackson, Marylen Rimando, Chad R. Edwards, Eveline Wu, Chris Cawley, Pamela D. EdwardsAnna Macgregor, Ryan Bozof, T. Michele Thompson, George J. Petro, Heather M. Shelton, Beth L. McCampbell, Jared C. Mills, Frederick L. Flynt, Timothy A. Steele, Marianne Kearney, Amy Kirincich-Greathead, Wade Hardy, Paul R. Young, Aman V. Amin, R. Steve Williams, Miranda M. Horton, Shaun McGuinn, Kristina C. Hawkins, Kurt Ericson, Louis Terracio, Catherine Moreau, Douglas Hixson, Brian W. Tobin, John Hudson, Frank P. Bowyer, Asa C. Black

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Undifferentiated cells have been identified in the prenatal blastocyst, inner cell mass, and gonadal ridges of rodents and primates, including humans. After isolation these cells express molecular and immunological markers for embryonic cells, capabilities for extended self-renewal, and telomerase activity. When allowed to differentiate, embryonic stem cells express phenotypic markers for tissues of ectodermal, mesodermal, and endodermal origin. When implanted in vivo, undifferentiated noninduced embryonic stem cells formed teratomas. In this report we describe a cell clone isolated from postnatal rat skeletal muscle and derived by repetitive single-cell clonogenic analysis. In the undifferentiated state it consists of very small cells having a high ratio of nucleus to cytoplasm. The clone expresses molecular and immunological markers for embryonic stem cells. It exhibits telomerase activity, which is consistent with its extended capability for self-renewal. When induced to differentiate, it expressed phenotypic markers for tissues of ectodermal, mesodermal, and endodermal origin. The clone was designated as a postnatal pluripotent epiblastic-like stem cell (PPELSC). The undifferentiated clone was transfected with a genomic marker and assayed for alterations in stem cell characteristics. No alterations were noted. The labeled clone, when implanted into heart after injury, incorporated into myocardial tissues undergoing repair. The labeled clone was subjected to directed lineage induction in vitro, resulting in the formation of islet-like structures (ILSs) that secreted insulin in response to a glucose challenge. This study suggests that embryonic-like stem cells are retained within postnatal mammals and have the potential for use in gene therapy and tissue engineering.

Original language	English (US)
Pages (from-to)	178-203
Number of pages	26
Journal	Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume	277
Issue number	1
DOIs	https://doi.org/10.1002/ar.a.20000
State	Published - Mar 2004

Keywords

Diabetes
Gene therapy
Myocardial infarction
Pluripotent stem cells

ASJC Scopus subject areas

Anatomy
Agricultural and Biological Sciences (miscellaneous)

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10.1002/ar.a.20000

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Young, H. E., Duplaa, C., Yost, M. J., Henson, N. L., Floyd, J. A., Detmer, K., Thompson, A. J., Powell, S. W., Gamblin, T. C., Kizziah, K., Holland, B. J., Boev, A., Van De Water, J. M., Godbee, D. C., Jackson, S., Rimando, M., Edwards, C. R., Wu, E., Cawley, C., ... Black, A. C. (2004). Clonogenic Analysis Reveals Reserve Stem Cells in Postnatal Mammals. II. Pluripotent Epiblastic-Like Stem Cells. Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, 277(1), 178-203. https://doi.org/10.1002/ar.a.20000

Clonogenic Analysis Reveals Reserve Stem Cells in Postnatal Mammals. II. Pluripotent Epiblastic-Like Stem Cells. / Young, Henry E.; Duplaa, Cecile; Yost, Michael J. et al.
In: Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, Vol. 277, No. 1, 03.2004, p. 178-203.

Research output: Contribution to journal › Article › peer-review

Young, HE, Duplaa, C, Yost, MJ, Henson, NL, Floyd, JA, Detmer, K, Thompson, AJ, Powell, SW, Gamblin, TC, Kizziah, K, Holland, BJ, Boev, A, Van De Water, JM, Godbee, DC, Jackson, S, Rimando, M, Edwards, CR, Wu, E, Cawley, C, Edwards, PD, Macgregor, A, Bozof, R, Thompson, TM, Petro, GJ, Shelton, HM, McCampbell, BL, Mills, JC, Flynt, FL, Steele, TA, Kearney, M, Kirincich-Greathead, A, Hardy, W, Young, PR, Amin, AV, Williams, RS, Horton, MM, McGuinn, S, Hawkins, KC, Ericson, K, Terracio, L, Moreau, C, Hixson, D, Tobin, BW, Hudson, J, Bowyer, FP & Black, AC 2004, 'Clonogenic Analysis Reveals Reserve Stem Cells in Postnatal Mammals. II. Pluripotent Epiblastic-Like Stem Cells', Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, vol. 277, no. 1, pp. 178-203. https://doi.org/10.1002/ar.a.20000

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abstract = "Undifferentiated cells have been identified in the prenatal blastocyst, inner cell mass, and gonadal ridges of rodents and primates, including humans. After isolation these cells express molecular and immunological markers for embryonic cells, capabilities for extended self-renewal, and telomerase activity. When allowed to differentiate, embryonic stem cells express phenotypic markers for tissues of ectodermal, mesodermal, and endodermal origin. When implanted in vivo, undifferentiated noninduced embryonic stem cells formed teratomas. In this report we describe a cell clone isolated from postnatal rat skeletal muscle and derived by repetitive single-cell clonogenic analysis. In the undifferentiated state it consists of very small cells having a high ratio of nucleus to cytoplasm. The clone expresses molecular and immunological markers for embryonic stem cells. It exhibits telomerase activity, which is consistent with its extended capability for self-renewal. When induced to differentiate, it expressed phenotypic markers for tissues of ectodermal, mesodermal, and endodermal origin. The clone was designated as a postnatal pluripotent epiblastic-like stem cell (PPELSC). The undifferentiated clone was transfected with a genomic marker and assayed for alterations in stem cell characteristics. No alterations were noted. The labeled clone, when implanted into heart after injury, incorporated into myocardial tissues undergoing repair. The labeled clone was subjected to directed lineage induction in vitro, resulting in the formation of islet-like structures (ILSs) that secreted insulin in response to a glucose challenge. This study suggests that embryonic-like stem cells are retained within postnatal mammals and have the potential for use in gene therapy and tissue engineering.",

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author = "Young, {Henry E.} and Cecile Duplaa and Yost, {Michael J.} and Henson, {Nicholas L.} and Floyd, {Julie A.} and Kristina Detmer and Thompson, {Angela J.} and Powell, {Steven W.} and Gamblin, {T. Clark} and Kirk Kizziah and Holland, {Benjamin J.} and Angel Boev and {Van De Water}, {J. M.} and Godbee, {Dan C.} and Stephanie Jackson and Marylen Rimando and Edwards, {Chad R.} and Eveline Wu and Chris Cawley and Edwards, {Pamela D.} and Anna Macgregor and Ryan Bozof and Thompson, {T. Michele} and Petro, {George J.} and Shelton, {Heather M.} and McCampbell, {Beth L.} and Mills, {Jared C.} and Flynt, {Frederick L.} and Steele, {Timothy A.} and Marianne Kearney and Amy Kirincich-Greathead and Wade Hardy and Young, {Paul R.} and Amin, {Aman V.} and Williams, {R. Steve} and Horton, {Miranda M.} and Shaun McGuinn and Hawkins, {Kristina C.} and Kurt Ericson and Louis Terracio and Catherine Moreau and Douglas Hixson and Tobin, {Brian W.} and John Hudson and Bowyer, {Frank P.} and Black, {Asa C.}",

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AU - Henson, Nicholas L.

AU - Floyd, Julie A.

AU - Detmer, Kristina

AU - Thompson, Angela J.

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AU - Kizziah, Kirk

AU - Holland, Benjamin J.

AU - Boev, Angel

AU - Van De Water, J. M.

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AU - Jackson, Stephanie

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AU - Edwards, Chad R.

AU - Wu, Eveline

AU - Cawley, Chris

AU - Edwards, Pamela D.

AU - Macgregor, Anna

AU - Bozof, Ryan

AU - Thompson, T. Michele

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AU - Shelton, Heather M.

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AU - Flynt, Frederick L.

AU - Steele, Timothy A.

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AU - Horton, Miranda M.

AU - McGuinn, Shaun

AU - Hawkins, Kristina C.

AU - Ericson, Kurt

AU - Terracio, Louis

AU - Moreau, Catherine

AU - Hixson, Douglas

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AU - Bowyer, Frank P.

AU - Black, Asa C.

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KW - Myocardial infarction

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Clonogenic Analysis Reveals Reserve Stem Cells in Postnatal Mammals. II. Pluripotent Epiblastic-Like Stem Cells

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Keywords

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