TY - JOUR
T1 - Closed-loop control of zebrafish behaviour in three dimensions using a robotic stimulus
AU - Kim, Changsu
AU - Ruberto, Tommaso
AU - Phamduy, Paul
AU - Porfiri, Maurizio
N1 - Funding Information:
This work was supported by the National Science Foundation under Grant # CMMI-1433670.
Funding Information:
This work was supported by the National Science Foundation under Grant # CMMI-1433670. The authors are grateful to Dr. Violet Mwaffo for useful discussion during the design and the fabrication of the robotic platform, and guidance during the data analyses of transfer entropy, and to Daniele Neri for his help in revising the manuscript.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Robotics is continuously being integrated in animal behaviour studies to create customizable, controllable, and repeatable stimuli. However, few systems have capitalized on recent breakthroughs in computer vision and real-time control to enable a two-way interaction between the animal and the robot. Here, we present a "closed-loop control" system to investigate the behaviour of zebrafish, a popular animal model in preclinical studies. The system allows for actuating a biologically-inspired 3D-printed replica in a 3D workspace, in response to the behaviour of a zebrafish. We demonstrate the role of closed-loop control in modulating the response of zebrafish, across a range of behavioural and information-theoretic measures. Our results suggest that closed-loop control could enhance the degree of biomimicry of the replica, by increasing the attraction of live subjects and their interaction with the stimulus. Interactive experiments hold promise to advance our understanding of zebrafish, offering new means for high throughput behavioural phenotyping.
AB - Robotics is continuously being integrated in animal behaviour studies to create customizable, controllable, and repeatable stimuli. However, few systems have capitalized on recent breakthroughs in computer vision and real-time control to enable a two-way interaction between the animal and the robot. Here, we present a "closed-loop control" system to investigate the behaviour of zebrafish, a popular animal model in preclinical studies. The system allows for actuating a biologically-inspired 3D-printed replica in a 3D workspace, in response to the behaviour of a zebrafish. We demonstrate the role of closed-loop control in modulating the response of zebrafish, across a range of behavioural and information-theoretic measures. Our results suggest that closed-loop control could enhance the degree of biomimicry of the replica, by increasing the attraction of live subjects and their interaction with the stimulus. Interactive experiments hold promise to advance our understanding of zebrafish, offering new means for high throughput behavioural phenotyping.
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U2 - 10.1038/s41598-017-19083-2
DO - 10.1038/s41598-017-19083-2
M3 - Article
C2 - 29330523
AN - SCOPUS:85040610155
SN - 2045-2322
VL - 8
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 657
ER -