Combined alcian blue and silver staining of glycosaminoglycans in polyacrylamide gels: Application to electrophoretic analysis of molecular weight distribution

Hoki Min, Mary K. Cowman

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Oligomeric and polymeric fragments of glycosaminoglycans may be separated for rapid analysis by electrophoresis through a 10% polyacrylamide matrix. A ladder-like series of bands is observed, in which adjacent major bands correspond to species differing in chain length by one disaccharide unit. The component species are detected by a combined alcian blue and silver staining protocol. Detection limits are less than 50 ng per band, or approximately 2-5 μg total load for polydisperse samples. Densitometry of the stained gel may be used to determine molecular weight averages and distribution. The applicable molecular weight ranges are approximately 4000 to 100,000 for hyaluronate, or 1500 to 40,000 for chondroitin and dermatan sulfate samples of moderate charge density heterogeneity.

    Original languageEnglish (US)
    Pages (from-to)275-285
    Number of pages11
    JournalAnalytical Biochemistry
    Volume155
    Issue number2
    DOIs
    StatePublished - Jun 1986

    Keywords

    • chondroitin sulfate
    • dermatan sulfate
    • electrophoresis
    • glycosaminoglycans
    • hyaluronate
    • staining

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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