Common features of polyomavirus mutants selected on PCC4 embryonal carcinoma cells.

F. Melin, H. Pinon, C. Reiss, C. Kress, N. Montreau, D. Blangy

Research output: Contribution to journalArticle

Abstract

The genomic rearrangements of six polyomavirus mutants selected on PCC4 embryonal carcinoma cells have been compared and their common characteristics pointed out. All mutants show a duplication which includes at least the adenovirus type 5 (Ad5) E1A-like enhancer core sequence plus a deletion of variable size and location. The presence of the second enhancer core sequence, the SV40-like enhancer, is not required for expression of the PyEC PCC4 phenotype. Two of these mutants are also able to express polyomavirus T antigen on F9 and LT1 cells. Multiadaptation seems to require the duplication of the Ad5 E1A-like core sequence, the maintenance of the SV40-like core sequence and a local change in DNA stability.

Original languageEnglish (US)
Pages (from-to)1799-1803
Number of pages5
JournalThe EMBO journal
Volume4
Issue number7
DOIs
StatePublished - Jul 1985

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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