Comparison of repair of methylated pyrimidines in poly(dT) by extracts from rat liver and Escherichia coli

M. Eileen Dolan; David Scicchitano; B. Singer; Anthony E. Pegg

doi:10.1016/0006-291X(84)90416-9

Comparison of repair of methylated pyrimidines in poly(dT) by extracts from rat liver and Escherichia coli

M. Eileen Dolan, David Scicchitano, B. Singer, Anthony E. Pegg

Research output: Contribution to journal › Article › peer-review

Abstract

Partially purified preparations of O⁶-alkylguanine-DNA alkyltransferase from rat liver and E. coli were tested for their ability to repair O⁴-methylthymine in a methylated poly(dT).poly(dA) substrate. The bacterial preparation readily carried out this reaction, but no loss of O⁴-methylthymine was obtained with the rat liver protein. These results indicate a significant difference in specificity between the mammalian and bacterial proteins which could have important consequences for carcinogenesis and mutagenesis by a alkylating agents in mammalian cells.

Original language	English (US)
Pages (from-to)	324-330
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	123
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(84)90416-9
State	Published - Aug 30 1984

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Partially purified preparations of O6-alkylguanine-DNA alkyltransferase from rat liver and E. coli were tested for their ability to repair O4-methylthymine in a methylated poly(dT).poly(dA) substrate. The bacterial preparation readily carried out this reaction, but no loss of O4-methylthymine was obtained with the rat liver protein. These results indicate a significant difference in specificity between the mammalian and bacterial proteins which could have important consequences for carcinogenesis and mutagenesis by a alkylating agents in mammalian cells.",

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AU - Singer, B.

AU - Pegg, Anthony E.

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N2 - Partially purified preparations of O6-alkylguanine-DNA alkyltransferase from rat liver and E. coli were tested for their ability to repair O4-methylthymine in a methylated poly(dT).poly(dA) substrate. The bacterial preparation readily carried out this reaction, but no loss of O4-methylthymine was obtained with the rat liver protein. These results indicate a significant difference in specificity between the mammalian and bacterial proteins which could have important consequences for carcinogenesis and mutagenesis by a alkylating agents in mammalian cells.

AB - Partially purified preparations of O6-alkylguanine-DNA alkyltransferase from rat liver and E. coli were tested for their ability to repair O4-methylthymine in a methylated poly(dT).poly(dA) substrate. The bacterial preparation readily carried out this reaction, but no loss of O4-methylthymine was obtained with the rat liver protein. These results indicate a significant difference in specificity between the mammalian and bacterial proteins which could have important consequences for carcinogenesis and mutagenesis by a alkylating agents in mammalian cells.

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Comparison of repair of methylated pyrimidines in poly(dT) by extracts from rat liver and Escherichia coli

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