Comparison of repair of methylated pyrimidines in poly(dT) by extracts from rat liver and Escherichia coli

M. Eileen Dolan, David Scicchitano, B. Singer, Anthony E. Pegg

Research output: Contribution to journalArticlepeer-review

Abstract

Partially purified preparations of O6-alkylguanine-DNA alkyltransferase from rat liver and E. coli were tested for their ability to repair O4-methylthymine in a methylated poly(dT).poly(dA) substrate. The bacterial preparation readily carried out this reaction, but no loss of O4-methylthymine was obtained with the rat liver protein. These results indicate a significant difference in specificity between the mammalian and bacterial proteins which could have important consequences for carcinogenesis and mutagenesis by a alkylating agents in mammalian cells.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume123
Issue number1
DOIs
StatePublished - Aug 30 1984

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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