Comparison of retinal nerve fiber layer thickness measurement bias and imprecision across three spectral-domain optical coherence tomography devices

Nancy M. Buchser, Gadi Wollstein, Hiroshi Ishikawa, Richard A. Bilonick, Yun Ling, Lindsey S. Folio, Larry Kagemann, Robert J. Noecker, Eiyass Albeiruti, Joel S. Schuman

    Research output: Contribution to journalArticle

    Abstract

    PURPOSE. We compared retinal nerve fiber layer (RNFL) bias and imprecision among three spectral-domain optical coherence tomographs (SD-OCT). METHODS. A total of 152 eyes of 83 subjects (96 healthy and 56 glaucomatous eyes) underwent peripapillary RNFL imaging using at least 2 of the following 3 SD-OCT devices on the same day: Cirrus HD-OCT (optic nerve head [ONH]) cube 200× 200 protocol), RTVue-100 (ONH protocol [12 radial lines and 13 concentric circles]), and 3D OCT-1000 (3D Scan 256 × 256 protocol). Calibration equations, bias and imprecision of RNFL measurements were calculated using structural equation models. RESULTS. The calibration equations for healthy and glaucoma RNFL thickness measurements among the 3 devices were: Cirrus = 2.136 + 0.831*RTVue; Cirrus 1/4 -15.521 {thorn} 1.056*3D OCT-1000; RTVue = -21.257 + 1.271*3D OCT-1000. Using Cirrus bias as an arbitrary reference, RTVue bias was 1.20 (95% CI 1.09-1.32, P < 0.05) times larger and 3D OCT-1000 was 0.95 (0.87-1.03, P > 0.05) times smaller. Relative to 3D OCT-1000, the RTVue bias was 1.27 (1.13-1.42, P < 0.05). RTVue imprecision (healthy eyes 7.83, 95% CI 6.43-9.58; glaucoma cases 5.71, 4.19-7.64) was statistically significantly higher than both Cirrus (healthy eyes 3.23, 2.11-4.31; glaucoma cases 3.53, 0.69-5.24) and 3D OCT-1000 (healthy eyes 4.07, 3.11-5.35; glaucoma cases 5.33, 3.77-7.67) in healthy eyes. The imprecision also was significantly higher for RTVue measurements in healthy compared to glaucomatous eyes. None of the other comparisons was statistically significant. CONCLUSIONS. RTVue-100 showed higher imprecision (or higher measurement variability) than Cirrus HD-OCT and 3D OCT- 1000 RNFL measurements. Three-dimensional cube scanning with post-hoc data sampling may be a factor reducing imprecision.

    Original languageEnglish (US)
    Pages (from-to)3742-3747
    Number of pages6
    JournalInvestigative Ophthalmology and Visual Science
    Volume53
    Issue number7
    DOIs
    StatePublished - Jun 2012

    ASJC Scopus subject areas

    • Ophthalmology
    • Sensory Systems
    • Cellular and Molecular Neuroscience

    Fingerprint Dive into the research topics of 'Comparison of retinal nerve fiber layer thickness measurement bias and imprecision across three spectral-domain optical coherence tomography devices'. Together they form a unique fingerprint.

  • Cite this

    Buchser, N. M., Wollstein, G., Ishikawa, H., Bilonick, R. A., Ling, Y., Folio, L. S., Kagemann, L., Noecker, R. J., Albeiruti, E., & Schuman, J. S. (2012). Comparison of retinal nerve fiber layer thickness measurement bias and imprecision across three spectral-domain optical coherence tomography devices. Investigative Ophthalmology and Visual Science, 53(7), 3742-3747. https://doi.org/10.1167/iovs.11-8432