TY - JOUR
T1 - Comparison of the nasopharynx microbiome between influenza and non-influenza cases of severe acute respiratory infections
T2 - A pilot study
AU - Borges, Luiz Gustavo dos Anjos
AU - Giongo, Adriana
AU - Pereira, Leandro de Mattos
AU - Trindade, Fernanda J.
AU - Gregianini, Tatiana Schäffer
AU - Campos, Fabrício Souza
AU - Ghedin, Elodie
AU - da Veiga, Ana Beatriz Gorini
N1 - Funding Information:
This study was supported by the Graduate Program in Pathology of UFCSPA, the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES‐MEC, Brazil), the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Edital 02/2014–PqG no. 2282–2551/14–5), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (Fellowship 311309/2012‐7 held by A.B.G.V.).
Funding Information:
This study was supported by the Graduate Program in Pathology of UFCSPA, the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES-MEC, Brazil), the Funda??o de Amparo ? Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Edital 02/2014?PqG no. 2282?2551/14?5), and the Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (Fellowship 311309/2012-7 held by A.B.G.V.). The authors acknowledge the High Performance Computing Lab?LAD/PUCRS for allowing access to run the sequencing analyses. We also thank Fernanda P. Valdez for technical assistance.
Publisher Copyright:
© 2018 The Authors. Health Science Reports published by Wiley Periodicals, Inc.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Aims: Influenza A virus (IAV) can cause severe acute respiratory infection (SARI), and disease outcome may be associated with changes in the microbiome of the nasopharynx. This is a pilot study to characterize the microbiome of the nasopharynx in patients hospitalized with SARI, infected and not infected by IAV. Methods and Results: Using target sequencing of the 16S rRNA gene, we assessed the bacterial community of nasopharyngeal aspirate samples and compared the microbiome of patients infected with IAV with the microbiome of patients who were negative for IAV. We observed differences in the relative abundance of Proteobacteria and Firmicutes between SARI patients, with Streptococcus being enriched and Pseudomonas underrepresented in IAV patients compared with patients who were not infected with IAV. Conclusion: Pseudomonas taxon seems to be in high frequency on the nasopharynx of SARI patients with non-IAV infection and might present a negative association with Streptococcus taxon. Microbial profile appears to be different between SARI patients infected or not infected with IAV.
AB - Aims: Influenza A virus (IAV) can cause severe acute respiratory infection (SARI), and disease outcome may be associated with changes in the microbiome of the nasopharynx. This is a pilot study to characterize the microbiome of the nasopharynx in patients hospitalized with SARI, infected and not infected by IAV. Methods and Results: Using target sequencing of the 16S rRNA gene, we assessed the bacterial community of nasopharyngeal aspirate samples and compared the microbiome of patients infected with IAV with the microbiome of patients who were negative for IAV. We observed differences in the relative abundance of Proteobacteria and Firmicutes between SARI patients, with Streptococcus being enriched and Pseudomonas underrepresented in IAV patients compared with patients who were not infected with IAV. Conclusion: Pseudomonas taxon seems to be in high frequency on the nasopharynx of SARI patients with non-IAV infection and might present a negative association with Streptococcus taxon. Microbial profile appears to be different between SARI patients infected or not infected with IAV.
KW - coinfection
KW - influenza A virus
KW - microbiome
KW - respiratory disease
KW - severe acute respiratory infection
KW - upper respiratory tract
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U2 - 10.1002/hsr2.47
DO - 10.1002/hsr2.47
M3 - Article
AN - SCOPUS:85056884299
SN - 2398-8835
VL - 1
JO - Health Science Reports
JF - Health Science Reports
IS - 6
M1 - e47
ER -