TY - JOUR
T1 - Comparison of the TIM and TOM channel activities of the mitochondrial protein import complexes
AU - Muro, Concepción
AU - Grigoriev, Serguei M.
AU - Pietkiewicz, Dawn
AU - Kinnally, Kathleen W.
AU - Campo, María Luisa
N1 - Funding Information:
This research was supported by National Science Foundation grants MCB9513439, MCB 9816950, and MCB0096206 (K.W.K.); National Institutes of Health grant GM57249 (K.W.K.); grant PB98-0988 from the Main Committee of Scientific and Technological Research of the Spanish Ministry of Education and Science; and Junta de Extremadura grants IPR00C041 and 2PR02B007 (M.L.C.). C.M. was a recipient of a PhD fellowship from the Spanish Ministry of Education.
PY - 2003/5/1
Y1 - 2003/5/1
N2 - Water-filled channels are central to the process of translocating proteins since they provide aqueous pathways through the hydrophobic environment of membranes. The Tom and Tim complexes translocate precursors across the mitochondrial outer and inner membranes, respectively, and contain channels referred to as TOM and TIM (previously called PSC and MCC). In this study, little differences were revealed from a direct comparison of the single channel properties of the TOM and TIM channels of yeast mitochondria. As they perform similar functions in translocating proteins across membranes, it is not surprising that both channels are high conductance, voltage-dependent channels that are slightly cation selective. Reconstituted TIM and TOM channel activities are not modified by deletion of the outer membrane channel VDAC, but are similarly affected by signal sequence peptides.
AB - Water-filled channels are central to the process of translocating proteins since they provide aqueous pathways through the hydrophobic environment of membranes. The Tom and Tim complexes translocate precursors across the mitochondrial outer and inner membranes, respectively, and contain channels referred to as TOM and TIM (previously called PSC and MCC). In this study, little differences were revealed from a direct comparison of the single channel properties of the TOM and TIM channels of yeast mitochondria. As they perform similar functions in translocating proteins across membranes, it is not surprising that both channels are high conductance, voltage-dependent channels that are slightly cation selective. Reconstituted TIM and TOM channel activities are not modified by deletion of the outer membrane channel VDAC, but are similarly affected by signal sequence peptides.
UR - http://www.scopus.com/inward/record.url?scp=0038637773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038637773&partnerID=8YFLogxK
U2 - 10.1016/S0006-3495(03)70024-1
DO - 10.1016/S0006-3495(03)70024-1
M3 - Article
C2 - 12719229
AN - SCOPUS:0038637773
SN - 0006-3495
VL - 84
SP - 2981
EP - 2989
JO - Biophysical journal
JF - Biophysical journal
IS - 5
ER -