TY - JOUR
T1 - Computer-based design of novel protein structures
AU - Butterfoss, Glenn L.
AU - Kuhlman, Brian
PY - 2006
Y1 - 2006
N2 - Over the past 10 years there has been tremendous success in the area of computational protein design. Protein design software has been used to stabilize proteins, solubilize membrane proteins, design intermolecular interactions, and design new protein structures. A key motivation for these studies is that they test our understanding of protein energetics and structure. De novo design of novel structures is a particularly rigorous test because the protein backbone must be designed in addition to the amino acid side chains. A priori it is not guaranteed that the target backbone is even designable. To address this issue, researchers have developed a variety of methods for generating protein-like scaffolds and for optimizing the protein backbone in conjunction with the amino acid sequence. These protocols have been used to design proteins from scratch and to explore sequence space for naturally occurring protein folds.
AB - Over the past 10 years there has been tremendous success in the area of computational protein design. Protein design software has been used to stabilize proteins, solubilize membrane proteins, design intermolecular interactions, and design new protein structures. A key motivation for these studies is that they test our understanding of protein energetics and structure. De novo design of novel structures is a particularly rigorous test because the protein backbone must be designed in addition to the amino acid side chains. A priori it is not guaranteed that the target backbone is even designable. To address this issue, researchers have developed a variety of methods for generating protein-like scaffolds and for optimizing the protein backbone in conjunction with the amino acid sequence. These protocols have been used to design proteins from scratch and to explore sequence space for naturally occurring protein folds.
KW - Computational protein design
KW - Flexible backbone design
KW - Molecular modeling
KW - Negative design
KW - Protein stability
UR - http://www.scopus.com/inward/record.url?scp=33745033790&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745033790&partnerID=8YFLogxK
U2 - 10.1146/annurev.biophys.35.040405.102046
DO - 10.1146/annurev.biophys.35.040405.102046
M3 - Review article
C2 - 16689627
AN - SCOPUS:33745033790
SN - 1056-8700
VL - 35
SP - 49
EP - 65
JO - Annual Review of Biophysics and Biomolecular Structure
JF - Annual Review of Biophysics and Biomolecular Structure
ER -