Concentration quenching of proflavine hydrochloride was studied in dry films of sodium deoxyribonucleate (DNA) and poly(vinyl alcohol) (PVA) by measuring the decline of flash-induced triplet formation with increasing dye concentration. The quencher was identified as the dimeric species of proflavine. The quenching of the excited singlet state may be interpreted in terms of long-range Förster-type energy-transfer mechanism. Critical donor-acceptor distances were found to be 33.5 and 46.3 Å in DNA and PVA, respectively; these differences may be explained by consideration of the polymer-dye interactions.
ASJC Scopus subject areas
- Physical and Theoretical Chemistry