Confocal microscopy of cardiac myocytes

Robert L. Price, Stephen T. Haley, Tara Bullard, Jeffrey Davis, Thomas K. Borg, Louis Terracio

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Detailed methods are provided for the preparation and confocal imaging of cardiac myocyte development and differentiation. Examples include protocols for the analysis of cultured myocytes as well as vibratome sections of hearts from embryonic and adult tissue. Techniques include routine labeling of F-actin with phalloidin as well as multiple labeling protocols for colocalization studies and cell volume analysis.

Original languageEnglish (US)
Title of host publicationConfocal Microscopy
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages185-199
Number of pages15
ISBN (Print)9781588293510
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1075
ISSN (Print)1064-3745

Keywords

  • Cardiac myocyte
  • Cardiovascular
  • Confocal microscopy
  • Image analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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