Conservation and diversification in homeodomain-DNA interactions: A comparative genetic analysis

David S. Wilson, Guojun Sheng, Susie Jun, Claude Desplan

Research output: Contribution to journalArticle

Abstract

Nearly all metazoan homeodomains (HDs) possess DNA binding targets that are related by the presence of a TAAT sequence. We use an in vitro genetic DNA binding site selection assay to refine our understanding of the amino acid determinants for the recognition of the TAAT site. Superimposed upon the conserved ability of metazoan HDs to recognize a TAAT core is a difference in their preference for the bases that lie immediately 3' to it. Amino acid position 50 of the HD has been shown to discriminate among these base pairs, and structural studies have suggested that water-mediated hydrogen bonds and van der Waals contacts underlie for this ability. Here, we show that each of six amino acids tested at position 50 can confer a distinct DNA binding specificity.

Original languageEnglish (US)
Pages (from-to)6886-6891
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number14
DOIs
StatePublished - Jul 9 1996

Keywords

  • DNA binding
  • evolution
  • structure
  • transcription factor

ASJC Scopus subject areas

  • General

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