TY - JOUR
T1 - Constitutive expression and regulation of collagenase-3 in human breast cancer cells
AU - Selvamurugan, Nagarajan
AU - Partridge, Nicola C.
N1 - Funding Information:
The authors thank K. M. Wiehl and O. Y. Barmina for their technical help and Dr. D. R. Tyson for reading the manuscript. This work was supported by a National Osteoporosis Foundation grant (to N.S.) and National Institutes of Health Grants DK47420 and DK48109 (to N.C.P.).
PY - 2000/4
Y1 - 2000/4
N2 - Matrix metalloproteinases (MMPs) are a family of secreted or transmembrane proteins that have been implicated in multiple physiological and pathological processes related to extracellular matrix turnover. Recent evidence strongly suggests a role for collagenase-3 (MMP-13) in tumor metastasis and invasion. We report here that collagenase-3 is constitutively expressed in the breast cancer cell line MDA-MB231 (MDA) and outline the molecular mechanism regulating its expression. Functional analysis of the collagenase-3 promoter showed that both the activator protein-1 (AP-1) site and the runt domain (RD) binding site were required for maximal constitutive expression of collagenase-3 in MDA cells. Determination of factors binding to those sites by Northern analysis and transient transfections identified the requirement of Fra-1, c-Jun, and Cbfa1 for basal collagenase-3 promoter activity in MDA cells. (C) 2000 Academic Press.
AB - Matrix metalloproteinases (MMPs) are a family of secreted or transmembrane proteins that have been implicated in multiple physiological and pathological processes related to extracellular matrix turnover. Recent evidence strongly suggests a role for collagenase-3 (MMP-13) in tumor metastasis and invasion. We report here that collagenase-3 is constitutively expressed in the breast cancer cell line MDA-MB231 (MDA) and outline the molecular mechanism regulating its expression. Functional analysis of the collagenase-3 promoter showed that both the activator protein-1 (AP-1) site and the runt domain (RD) binding site were required for maximal constitutive expression of collagenase-3 in MDA cells. Determination of factors binding to those sites by Northern analysis and transient transfections identified the requirement of Fra-1, c-Jun, and Cbfa1 for basal collagenase-3 promoter activity in MDA cells. (C) 2000 Academic Press.
KW - Activator protein-1
KW - Collagenase- 3
KW - Core binding factor
KW - Matrix metalloproteinase
KW - Tumor metastasis and invasion
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U2 - 10.1006/mcbr.2000.0215
DO - 10.1006/mcbr.2000.0215
M3 - Article
C2 - 10891395
AN - SCOPUS:0033862549
SN - 1522-4724
VL - 3
SP - 218
EP - 223
JO - Molecular Cell Biology Research Communications
JF - Molecular Cell Biology Research Communications
IS - 4
ER -