Constitutive expression and regulation of collagenase-3 in human breast cancer cells

Nagarajan Selvamurugan, Nicola C. Partridge

Research output: Contribution to journalArticlepeer-review

Abstract

Matrix metalloproteinases (MMPs) are a family of secreted or transmembrane proteins that have been implicated in multiple physiological and pathological processes related to extracellular matrix turnover. Recent evidence strongly suggests a role for collagenase-3 (MMP-13) in tumor metastasis and invasion. We report here that collagenase-3 is constitutively expressed in the breast cancer cell line MDA-MB231 (MDA) and outline the molecular mechanism regulating its expression. Functional analysis of the collagenase-3 promoter showed that both the activator protein-1 (AP-1) site and the runt domain (RD) binding site were required for maximal constitutive expression of collagenase-3 in MDA cells. Determination of factors binding to those sites by Northern analysis and transient transfections identified the requirement of Fra-1, c-Jun, and Cbfa1 for basal collagenase-3 promoter activity in MDA cells. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)218-223
Number of pages6
JournalMolecular Cell Biology Research Communications
Volume3
Issue number4
DOIs
StatePublished - Apr 2000

Keywords

  • Activator protein-1
  • Collagenase- 3
  • Core binding factor
  • Matrix metalloproteinase
  • Tumor metastasis and invasion

ASJC Scopus subject areas

  • Molecular Biology

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