Construction and use of an electrochemical NO sensor in a cell-based assessing system

Ken Ichiro Kamei, Masayasu Mie, Yasuko Yanagida, Masuo Aizawa, Eiry Kobatake

Research output: Contribution to journalArticlepeer-review


We have constructed a cell-based assessing system for chemicals using a probe-type electrochemical nitric oxide (NO) sensor. This system can be used to assess the effects of chemicals on the nitric oxide signaling pathway in immune systems. NO was used as an indicator of a cellular immune response due to chemical exposure. RAW264.7 cells, a macrophage-like cell line, were used as a model immune cell. The novel probe-type electrochemical NO sensor, consisting of a Pt counter electrode, an Ag/AgCl reference electrode, and a gold electrode coated with a polyion complex layer, was constructed for this study. The NO sensor probe measures NO without the drawback of electrochemical interference. The detection limit of the sensor was 8.4nM NO. The cell-based system was able to assess the immuno-activating effects of lipopolysaccharide and interferon-γ, and the inhibiting effects of L-NMMA in a dose-dependent manner.

Original languageEnglish (US)
Pages (from-to)106-112
Number of pages7
JournalSensors and Actuators, B: Chemical
Issue number1
StatePublished - Apr 15 2004


  • Cell-based assessing system
  • Inducible nitric oxide synthase
  • Nitric oxide
  • Polyion complex

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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