Construction and use of an electrochemical NO sensor in a cell-based assessing system

Ken Ichiro Kamei; Masayasu Mie; Yasuko Yanagida; Masuo Aizawa; Eiry Kobatake

doi:10.1016/j.snb.2003.11.003

Construction and use of an electrochemical NO sensor in a cell-based assessing system

Ken Ichiro Kamei, Masayasu Mie, Yasuko Yanagida, Masuo Aizawa, Eiry Kobatake

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We have constructed a cell-based assessing system for chemicals using a probe-type electrochemical nitric oxide (NO) sensor. This system can be used to assess the effects of chemicals on the nitric oxide signaling pathway in immune systems. NO was used as an indicator of a cellular immune response due to chemical exposure. RAW264.7 cells, a macrophage-like cell line, were used as a model immune cell. The novel probe-type electrochemical NO sensor, consisting of a Pt counter electrode, an Ag/AgCl reference electrode, and a gold electrode coated with a polyion complex layer, was constructed for this study. The NO sensor probe measures NO without the drawback of electrochemical interference. The detection limit of the sensor was 8.4nM NO. The cell-based system was able to assess the immuno-activating effects of lipopolysaccharide and interferon-γ, and the inhibiting effects of L-NMMA in a dose-dependent manner.

Original language	English (US)
Pages (from-to)	106-112
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	99
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2003.11.003
State	Published - Apr 15 2004

Keywords

Cell-based assessing system
Inducible nitric oxide synthase
Nitric oxide
Polyion complex

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2003.11.003

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title = "Construction and use of an electrochemical NO sensor in a cell-based assessing system",

abstract = "We have constructed a cell-based assessing system for chemicals using a probe-type electrochemical nitric oxide (NO) sensor. This system can be used to assess the effects of chemicals on the nitric oxide signaling pathway in immune systems. NO was used as an indicator of a cellular immune response due to chemical exposure. RAW264.7 cells, a macrophage-like cell line, were used as a model immune cell. The novel probe-type electrochemical NO sensor, consisting of a Pt counter electrode, an Ag/AgCl reference electrode, and a gold electrode coated with a polyion complex layer, was constructed for this study. The NO sensor probe measures NO without the drawback of electrochemical interference. The detection limit of the sensor was 8.4nM NO. The cell-based system was able to assess the immuno-activating effects of lipopolysaccharide and interferon-γ, and the inhibiting effects of L-NMMA in a dose-dependent manner.",

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AU - Mie, Masayasu

AU - Yanagida, Yasuko

AU - Aizawa, Masuo

AU - Kobatake, Eiry

PY - 2004/4/15

Y1 - 2004/4/15

N2 - We have constructed a cell-based assessing system for chemicals using a probe-type electrochemical nitric oxide (NO) sensor. This system can be used to assess the effects of chemicals on the nitric oxide signaling pathway in immune systems. NO was used as an indicator of a cellular immune response due to chemical exposure. RAW264.7 cells, a macrophage-like cell line, were used as a model immune cell. The novel probe-type electrochemical NO sensor, consisting of a Pt counter electrode, an Ag/AgCl reference electrode, and a gold electrode coated with a polyion complex layer, was constructed for this study. The NO sensor probe measures NO without the drawback of electrochemical interference. The detection limit of the sensor was 8.4nM NO. The cell-based system was able to assess the immuno-activating effects of lipopolysaccharide and interferon-γ, and the inhibiting effects of L-NMMA in a dose-dependent manner.

AB - We have constructed a cell-based assessing system for chemicals using a probe-type electrochemical nitric oxide (NO) sensor. This system can be used to assess the effects of chemicals on the nitric oxide signaling pathway in immune systems. NO was used as an indicator of a cellular immune response due to chemical exposure. RAW264.7 cells, a macrophage-like cell line, were used as a model immune cell. The novel probe-type electrochemical NO sensor, consisting of a Pt counter electrode, an Ag/AgCl reference electrode, and a gold electrode coated with a polyion complex layer, was constructed for this study. The NO sensor probe measures NO without the drawback of electrochemical interference. The detection limit of the sensor was 8.4nM NO. The cell-based system was able to assess the immuno-activating effects of lipopolysaccharide and interferon-γ, and the inhibiting effects of L-NMMA in a dose-dependent manner.

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KW - Inducible nitric oxide synthase

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KW - Polyion complex

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Construction and use of an electrochemical NO sensor in a cell-based assessing system

Abstract

Keywords

ASJC Scopus subject areas

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