Construction, expression and recognition of an H - 2 molecule lacking its carboxyl terminus

Cornells Murre, Carol S. Reiss, Carmelo Bernabeu, Lan Bo Chen, Steven J. Burakoff, J. G. Seidman

Research output: Contribution to journalArticle

Abstract

A mouse major histocompatibility antigen (H-2) gene, encoding a novel H-2Ld molecule lacking its intracytoplasmic domain, has been constructed and introduced into mouse L-cells. The novel H-2 molecule is found on the surface of the transfected cells at the same level as L-cells transfected with the native H-2Ld gene. Allo- and influenza-specific cytotoxic T lymphocytes can recognize the truncated H-2 gene product nearly as efficiently as the normal H-2Ld gene product. However, vesicular stomatitis virus-specific cytotoxic T lymphocytes recognize the truncated H-2Ld molecule less efficiently than the complete H-2Ld product. The rate of capping of the truncated H-2Ld molecule was investigated and found to be the same as that of the complete H-2Ld gene product.

Original languageEnglish (US)
Pages (from-to)432-436
Number of pages5
JournalNature
Volume307
Issue number5950
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • General

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