Context-dependent neocentromere activity in synthetic yeast chromosome VIII

The Build-A-Genome Class

Research output: Contribution to journalArticlepeer-review


Pioneering advances in genome engineering, and specifically in genome writing, have revolutionized the field of synthetic biology, propelling us toward the creation of synthetic genomes. The Sc2.0 project aims to build the first fully synthetic eukaryotic organism by assembling the genome of Saccharomyces cerevisiae. With the completion of synthetic chromosome VIII (synVIII) described here, this goal is within reach. In addition to writing the yeast genome, we sought to manipulate an essential functional element: the point centromere. By relocating the native centromere sequence to various positions along chromosome VIII, we discovered that the minimal 118-bp CEN8 sequence is insufficient for conferring chromosomal stability at ectopic locations. Expanding the transplanted sequence to include a small segment (∼500 bp) of the CDEIII-proximal pericentromere improved chromosome stability, demonstrating that minimal centromeres display context-dependent functionality.

Original languageEnglish (US)
Article number100437
JournalCell Genomics
Issue number11
StatePublished - Nov 8 2023


  • Saccharomyces cerevisiae
  • aneuploidy
  • centromere
  • chromosomal stability
  • genome engineering
  • genome rearrangements
  • pericentromere
  • synVIII

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Genetics


Dive into the research topics of 'Context-dependent neocentromere activity in synthetic yeast chromosome VIII'. Together they form a unique fingerprint.

Cite this