Continuous-flow pi-based sorting of proteins and peptides for isolation of basic pi range molecules

Yong Ak Song, Chris Celio, Jongyoon Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel continuous-flow isoelectric point (pI)-based sorting technique has been developed for proteins and peptides in PDMS microfluidic chip. As a free-flow electrophoresis, it can continuously sort the biomolecules at a relatively high flow rate up to 5PL/min. The electrophoretic field required for the separation of biomolecules can be generated either by the diffusion potential or by an external power source in combination with the diffusion potential field. As for applying an external electric field, we have developed a simple patterning technique to generate a nanogap junction between the main sorting channel and the electrode reservoirs. This junction allows an effective integration of the external electric field into the sorting channel. Using the hybrid sorting technique, we could demonstrate a pI-based sorting of proteins and peptides even in basic pH ranges.

Original languageEnglish (US)
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages811-813
Number of pages3
ISBN (Electronic)9780979806407
StatePublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: Oct 7 2007Oct 11 2007

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
CountryFrance
CityParis
Period10/7/0710/11/07

Keywords

  • Free-Flow Electrophoresis
  • Peptides
  • Proteins
  • Separation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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