@inproceedings{6e338cdc4d7f440c8cd77fcded9acb2f,
title = "Continuous-flow pi-based sorting of proteins and peptides for isolation of basic pi range molecules",
abstract = "A novel continuous-flow isoelectric point (pI)-based sorting technique has been developed for proteins and peptides in PDMS microfluidic chip. As a free-flow electrophoresis, it can continuously sort the biomolecules at a relatively high flow rate up to 5PL/min. The electrophoretic field required for the separation of biomolecules can be generated either by the diffusion potential or by an external power source in combination with the diffusion potential field. As for applying an external electric field, we have developed a simple patterning technique to generate a nanogap junction between the main sorting channel and the electrode reservoirs. This junction allows an effective integration of the external electric field into the sorting channel. Using the hybrid sorting technique, we could demonstrate a pI-based sorting of proteins and peptides even in basic pH ranges.",
keywords = "Free-Flow Electrophoresis, Peptides, Proteins, Separation",
author = "Song, {Yong Ak} and Chris Celio and Jongyoon Han",
note = "Publisher Copyright: {\textcopyright} 2007 CBMS.; 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 ; Conference date: 07-10-2007 Through 11-10-2007",
year = "2007",
language = "English (US)",
series = "Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007",
publisher = "Chemical and Biological Microsystems Society",
pages = "811--813",
editor = "Jean-Louis Viovy and Patrick Tabeling and Stephanie Descroix and Laurent Malaquin",
booktitle = "Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007",
}