Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential

Yong Ak Song, Jongyoon Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel continuous pI-based sorting technique has been developed for proteins and peptides. It can continuously process samples at a relatively high flow rate of 10L/min and requires no ampholytes. The electrophoretic field required for the separation of biomolecules is generated by the diffusion of different buffer ionic species in-situ at the laminar flow junction. Using this diffusion potential, a pI-based separation of proteins and peptides has been successfully demonstrated. The resolution of the sorting technique and its efficiency were characterized by fluorescence intensity measurement, as well as by 2D protein gel electrophoresis.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1025-1027
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • Continuous freeflow electrophoresis
  • Diffusion potential
  • Protein separation
  • Transverse IEF

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Song, Y. A., & Han, J. (2005). Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1025-1027). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.