Control of mitochondrial protein import by pH

Sergey M. Grigoriev, Robert E. Jensen, Kathleen W. Kinnally

    Research output: Contribution to journalArticlepeer-review


    Protein import into mitochondria is inhibited by protons (IC50 pH 6.5). The channels of the import machinery were examined to further investigate this pH dependence. TOM and TIM23 are the protein translocation channels of the mitochondrial outer and inner membranes, respectively, and their single channel behaviors at various pHs were determined using patch-clamp techniques. While not identical, increasing H+ concentration decreases the open probability of both TIM23 and TOM channels. The pattern of the pH dependences of protein import and channel properties suggests TIM23 open probability can limit import of nuclear-encoded proteins into the matrix of yeast mitochondria.

    Original languageEnglish (US)
    Pages (from-to)163-166
    Number of pages4
    JournalFEBS Letters
    Issue number1-2
    StatePublished - Oct 9 2003


    • Mitochondrion
    • PH
    • Patch clamp
    • Protein import
    • Translocase of inner membrane
    • Translocase of outer membrane

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology


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