TY - JOUR
T1 - Cooperation between a hierarchical set of recruitment sites targets the X chromosome for dosage compensation
AU - Albritton, Sarah Elizabeth
AU - Kranz, Anna Lena
AU - Winterkorn, Lara Heermans
AU - Street, Lena Annika
AU - Ercan, Sevinc
N1 - Funding Information:
We thank NYU-CGSB High Throughput Sequencing Facility for sequencing and raw data processing. We thank Knudra Trangenics, Cecilia Pellegrini and Sofija Miljovska for their contribution to making strains. Some strains were provided by the CGC, which is funded by NIH Office of Research Infra-structure Programs (P40 OD010440). Research reported in this publication was supported by NIGMS of the National Institutes of Health under award number R01GM107293.
PY - 2017/5/30
Y1 - 2017/5/30
N2 - In many organisms, it remains unclear how X chromosomes are specified for dosage compensation, since DNA sequence motifs shown to be important for dosage compensation complex (DCC) recruitment are themselves not X-specific. Here, we addressed this problem in C. elegans. We found that the DCC recruiter, SDC-2, is required to maintain open chromatin at a small number of primary DCC recruitment sites, whose sequence and genomic context are X-specific. Along the X, primary recruitment sites are interspersed with secondary sites, whose function is X-dependent. A secondary site can ectopically recruit the DCC when additional recruitment sites are inserted either in tandem or at a distance (>30 kb). Deletion of a recruitment site on the X results in reduced DCC binding across several megabases surrounded by topologically associating domain (TAD) boundaries. Our work elucidates that hierarchy and long-distance cooperativity between gene-regulatory elements target a single chromosome for regulation.
AB - In many organisms, it remains unclear how X chromosomes are specified for dosage compensation, since DNA sequence motifs shown to be important for dosage compensation complex (DCC) recruitment are themselves not X-specific. Here, we addressed this problem in C. elegans. We found that the DCC recruiter, SDC-2, is required to maintain open chromatin at a small number of primary DCC recruitment sites, whose sequence and genomic context are X-specific. Along the X, primary recruitment sites are interspersed with secondary sites, whose function is X-dependent. A secondary site can ectopically recruit the DCC when additional recruitment sites are inserted either in tandem or at a distance (>30 kb). Deletion of a recruitment site on the X results in reduced DCC binding across several megabases surrounded by topologically associating domain (TAD) boundaries. Our work elucidates that hierarchy and long-distance cooperativity between gene-regulatory elements target a single chromosome for regulation.
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U2 - 10.7554/eLife.23645
DO - 10.7554/eLife.23645
M3 - Article
C2 - 28562241
AN - SCOPUS:85020456051
VL - 6
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e23645
ER -