TY - JOUR
T1 - Coordination between stochastic and deterministic specification in the Drosophila visual system
AU - Courgeon, Maximilien
AU - Desplan, Claude
N1 - Funding Information:
We thank the fly community, the Bloomington and Kyoto Stock centers, and the DGRC for sharing flies and reagents; M. Wernet, L. Venkatasubramanian, K. Menon, and K. Zinn for sharing data prior to publication; B. Johnston and L. Zipursky for unpublished data and fly stocks; C.-H. Lee, L. Venkatasubramanian, R. Mann, and H. Bellen for fly strains; D. Godt and Y.-N. Jan for antibodies; J. Treisman, E. Mazzoni, and N. Ringstad for their input during the investigation; and all Desplan lab members for their discussion and comments on the manuscript. Supported by NIH grant R01 EY13010 (C.D.).
Publisher Copyright:
Copyright © 2019 The Authors,
PY - 2019/10/18
Y1 - 2019/10/18
N2 - Sensory systems use stochastic fate specification to increase their repertoire of neuronal types. How these stochastic decisions are coordinated with the development of their targets is unknown. In the Drosophila retina, two subtypes of ultraviolet-sensitive R7 photoreceptors are stochastically specified. In contrast, their targets in the brain are specified through a deterministic program. We identified subtypes of the main target of R7, the Dm8 neurons, each specific to the different subtypes of R7s. Dm8 subtypes are produced in excess by distinct neuronal progenitors, independently from R7. After matching with their cognate R7, supernumerary Dm8s are eliminated by apoptosis. Two interacting cell adhesion molecules, Dpr11 and DIPg, are essential for the matching of one of the synaptic pairs. These mechanisms allow the qualitative and quantitative matching of R7 and Dm8 and thereby permit the stochastic choice made in R7 to propagate to the brain.
AB - Sensory systems use stochastic fate specification to increase their repertoire of neuronal types. How these stochastic decisions are coordinated with the development of their targets is unknown. In the Drosophila retina, two subtypes of ultraviolet-sensitive R7 photoreceptors are stochastically specified. In contrast, their targets in the brain are specified through a deterministic program. We identified subtypes of the main target of R7, the Dm8 neurons, each specific to the different subtypes of R7s. Dm8 subtypes are produced in excess by distinct neuronal progenitors, independently from R7. After matching with their cognate R7, supernumerary Dm8s are eliminated by apoptosis. Two interacting cell adhesion molecules, Dpr11 and DIPg, are essential for the matching of one of the synaptic pairs. These mechanisms allow the qualitative and quantitative matching of R7 and Dm8 and thereby permit the stochastic choice made in R7 to propagate to the brain.
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U2 - 10.1126/science.aay6727
DO - 10.1126/science.aay6727
M3 - Article
C2 - 31582524
AN - SCOPUS:85073576523
VL - 366
JO - Science
JF - Science
SN - 0036-8075
IS - 6463
M1 - eaay6727
ER -