Correlative light and backscattered electron microscopy of bone - Part II: Automated image analysis

Haviva M. Goldman, Aron Blayvas, Alan Boyde, Peter G.T. Howell, John G. Clement, Timothy G. Bromage

Research output: Contribution to journalArticlepeer-review


Detailed studies of biological phenomena often involve multiple microscopy and imaging modes and media. For bone biology, various forms of light and electron microscopy are used to study the microscopic structure of bone. Integrating information from the different sources is necessary to understand how different aspects of the bone structure interact. To accomplish this, methods were developed to prepare and image thin sections for correlative light microscopy (LM) and backscattered electron imaging in the scanning electron microscope (BSE-SEM). Images of the same fields of view may then be analyzed for degrees of relationships between specimen features not observed by LM or SEM alone. These methods are applied here to study possible associations between the degree of bone mineralization and pattern of collagen fiber orientation in the mid-shaft of the human femur. The "relational images" obtained allow us to examine the relationship between these two variables, both objectively and quantitatively.

Original languageEnglish (US)
Pages (from-to)337-344
Number of pages8
Issue number6
StatePublished - Jan 1 2000


  • Circularly polarized light
  • Human mid-shaft femur
  • Image analysis
  • Image comparison
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation


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