Correlative light and backscattered electron microscopy of bone - Part I: Specimen preparation methods

Haviva M. Goldman, John Kindsvater, Timothy G. Bromage

Research output: Contribution to journalArticle

Abstract

A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.

Original languageEnglish (US)
Pages (from-to)40-43
Number of pages4
JournalScanning
Volume21
Issue number1
DOIs
StatePublished - 1999

Keywords

  • Backscattered electron microscopy
  • Correlative studies
  • Light cure dental adhesives
  • Light microscopy
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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