A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.
- Backscattered electron microscopy
- Correlative studies
- Light cure dental adhesives
- Light microscopy
- Scanning electron microscopy
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics