Correlative light and backscattered electron microscopy of bone - Part I: Specimen preparation methods

Haviva M. Goldman; John Kindsvater; Timothy G. Bromage

doi:10.1002/sca.4950210106

Correlative light and backscattered electron microscopy of bone - Part I: Specimen preparation methods

Haviva M. Goldman, John Kindsvater, Timothy G. Bromage

Research output: Contribution to journal › Article › peer-review

Abstract

A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.

Original language	English (US)
Pages (from-to)	40-43
Number of pages	4
Journal	Scanning
Volume	21
Issue number	1
DOIs	https://doi.org/10.1002/sca.4950210106
State	Published - 1999

Keywords

Backscattered electron microscopy
Correlative studies
Light cure dental adhesives
Light microscopy
Scanning electron microscopy

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Instrumentation

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10.1002/sca.4950210106

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title = "Correlative light and backscattered electron microscopy of bone - Part I: Specimen preparation methods",

abstract = "A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.",

keywords = "Backscattered electron microscopy, Correlative studies, Light cure dental adhesives, Light microscopy, Scanning electron microscopy",

author = "Goldman, {Haviva M.} and John Kindsvater and Bromage, {Timothy G.}",

year = "1999",

doi = "10.1002/sca.4950210106",

language = "English (US)",

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Y1 - 1999

N2 - A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.

AB - A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (BSE-SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light-cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.

KW - Backscattered electron microscopy

KW - Correlative studies

KW - Light cure dental adhesives

KW - Light microscopy

KW - Scanning electron microscopy

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Correlative light and backscattered electron microscopy of bone - Part I: Specimen preparation methods

Abstract

Keywords

ASJC Scopus subject areas

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