TY - JOUR
T1 - Cost and Time Effective Lithography of Reusable Millimeter Size Bone Tissue Replicas With Sub-15 nm Feature Size on A Biocompatible Polymer
AU - Liu, Xiangyu
AU - Zanut, Alessandra
AU - Sladkova-Faure, Martina
AU - Xie, Liyuan
AU - Weck, Marcus
AU - Zheng, Xiaorui
AU - Riedo, Elisa
AU - de Peppo, Giuseppe Maria
N1 - Funding Information:
X.L. and A.Z. contributed equally to this work. The experiments were performed with the NanoFrazor, acquired through the NSF CMMI MRI – 1929453 grant. The authors acknowledge support from the US Army Research Office, the Office of Basic Energy Sciences of the US Department of Energy, the National Science Foundation (CMMI and CBET programs), the New York Stem Cell Foundation Research Institute, and the Ralph and Ricky Lauren Family Foundation. This work was also partially supported by the MRSEC Program of the National Science Foundation under Award Number DMR‐1420073. The authors thank Prof. Joseph Wallace of Purdue University for the AFM bone image reported in Figure 3a . The authors also thank Drs. Rick Monsma and Raeka Aiyar, and Corvis Richardson for proofreading the manuscript, and the staff at the City University of New York's Advanced Science Research Center for their help with SEM analysis.
Funding Information:
X.L. and A.Z. contributed equally to this work. The experiments were performed with the NanoFrazor, acquired through the NSF CMMI MRI ? 1929453 grant. The authors acknowledge support from the US Army Research Office, the Office of Basic Energy Sciences of the US Department of Energy, the National Science Foundation (CMMI and CBET programs), the New York Stem Cell Foundation Research Institute, and the Ralph and Ricky Lauren Family Foundation. This work was also partially supported by the MRSEC Program of the National Science Foundation under Award Number DMR-1420073. The authors thank Prof. Joseph Wallace of Purdue University for the AFM bone image reported in Figure?3a. The authors also thank Drs. Rick Monsma and Raeka Aiyar, and Corvis Richardson for proofreading the manuscript, and the staff at the City University of New York's Advanced Science Research Center for their help with SEM analysis.
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021
Y1 - 2021
N2 - The ability to replicate the microenvironment of biological tissues creates unique biomedical possibilities for stem cell applications. Current fabrication methods are limited by either the control on feature size and shape, or by the throughput and size of the replicas. Here, a novel platform is reported that combines thermal scanning probe lithography (tSPL) with innovative methodologies for the low-cost and high-throughput nanofabrication of large area quasi-3D bone tissue replicas with high fidelity, sub-15 nm lateral precision, and sub-2 nm vertical resolution. This bio-tSPL platform features a biocompatible polymer resist that withstands multiple cell culture cycles, allowing the reuse of the replicas, further decreasing costs and fabrication times. The as-fabricated replicas support the culture and proliferation of human induced mesenchymal stem cells, which display broad therapeutic and biomedical potential. Furthermore, it is demonstrated that bio-tSPL can be used to nanopattern the bone tissue replicas with amine groups, for subsequent tissue-mimetic biofunctionalization. The achieved level of time and cost-effectiveness, as well as the cell compatibility of the replicas, make bio-tSPL a promising platform for the production of tissue-mimetic replicas to study stem cell-tissue microenvironment interactions, test drugs, and ultimately harness the regenerative capacity of stem cells and tissues for biomedical applications.
AB - The ability to replicate the microenvironment of biological tissues creates unique biomedical possibilities for stem cell applications. Current fabrication methods are limited by either the control on feature size and shape, or by the throughput and size of the replicas. Here, a novel platform is reported that combines thermal scanning probe lithography (tSPL) with innovative methodologies for the low-cost and high-throughput nanofabrication of large area quasi-3D bone tissue replicas with high fidelity, sub-15 nm lateral precision, and sub-2 nm vertical resolution. This bio-tSPL platform features a biocompatible polymer resist that withstands multiple cell culture cycles, allowing the reuse of the replicas, further decreasing costs and fabrication times. The as-fabricated replicas support the culture and proliferation of human induced mesenchymal stem cells, which display broad therapeutic and biomedical potential. Furthermore, it is demonstrated that bio-tSPL can be used to nanopattern the bone tissue replicas with amine groups, for subsequent tissue-mimetic biofunctionalization. The achieved level of time and cost-effectiveness, as well as the cell compatibility of the replicas, make bio-tSPL a promising platform for the production of tissue-mimetic replicas to study stem cell-tissue microenvironment interactions, test drugs, and ultimately harness the regenerative capacity of stem cells and tissues for biomedical applications.
KW - bone microenvironment
KW - human induced pluripotent stem cells
KW - nanofabrication
KW - thermal scanning probe lithography
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85100452730&partnerID=8YFLogxK
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U2 - 10.1002/adfm.202008662
DO - 10.1002/adfm.202008662
M3 - Article
AN - SCOPUS:85100452730
SN - 1616-301X
VL - 31
JO - Advanced Functional Materials
JF - Advanced Functional Materials
IS - 19
M1 - 2008662
ER -