CRISPR knockouts in Ciona embryos

Shashank Gandhi; Florian Razy-Krajka; Lionel Christiaen; Alberto Stolfi

doi:10.1007/978-981-10-7545-2_13

CRISPR knockouts in Ciona embryos

Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi

Biology and Genomics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage.

Original language	English (US)
Title of host publication	Advances in Experimental Medicine and Biology
Publisher	Springer New York LLC
Pages	141-152
Number of pages	12
DOIs	https://doi.org/10.1007/978-981-10-7545-2_13
State	Published - 2018

Publication series

Name	Advances in Experimental Medicine and Biology
Volume	1029
ISSN (Print)	0065-2598
ISSN (Electronic)	2214-8019

Keywords

Chordates
Genome editing
Somatic gene knockout
Targeted mutagenesis
Tunicates
sgRNAs

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1007/978-981-10-7545-2_13

Cite this

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abstract = "Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage.",

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doi = "10.1007/978-981-10-7545-2_13",

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AU - Razy-Krajka, Florian

AU - Christiaen, Lionel

AU - Stolfi, Alberto

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N2 - Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage.

AB - Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage.

KW - Chordates

KW - Genome editing

KW - Somatic gene knockout

KW - Targeted mutagenesis

KW - Tunicates

KW - sgRNAs

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BT - Advances in Experimental Medicine and Biology

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ER -

CRISPR knockouts in Ciona embryos

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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