CRISPR knockouts in Ciona embryos

Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages141-152
Number of pages12
DOIs
StatePublished - 2018

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1029
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Chordates
  • Genome editing
  • Somatic gene knockout
  • Targeted mutagenesis
  • Tunicates
  • sgRNAs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Gandhi, S., Razy-Krajka, F., Christiaen, L., & Stolfi, A. (2018). CRISPR knockouts in Ciona embryos. In Advances in Experimental Medicine and Biology (pp. 141-152). (Advances in Experimental Medicine and Biology; Vol. 1029). Springer New York LLC. https://doi.org/10.1007/978-981-10-7545-2_13