Crystal structure of a naturally occurring dinucleoside phosphate: Uridylyl 3′,5′-adenosine phosphate model for RNA chain folding

Joel L. Sussman, Nadrian C. Seeman, S. H. Kim, Helen M. Berman

Research output: Contribution to journalArticlepeer-review

Abstract

The crystal structure of uridylyl 3′,5′-adenosine phosphate (UpA) has been determined and refined to an R value of 0.062. There are two UpA molecules per asymmetric unit in a monoclinic unit cell with dimensions a = 16.91, b = 12.37, c = 11.25 A ̊ and β = 96.0 °. The conformation of the phosphodiester linkage is different for the two molecules; one UpA suggests a model for a sharp turn in a single-stranded nucleic acid structure and the other for a helical structure. All the nucleoside units have similar conformations. There is no complementary base pairing between the adenines and uracils but rather layers of base-paired adenines and base-paired uracils separated by 3.4 Å. The predominant form of base stacking involves the ribosyl oxygen atom and the bases.

Original languageEnglish (US)
Pages (from-to)403-421
Number of pages19
JournalJournal of Molecular Biology
Volume66
Issue number3
DOIs
StatePublished - May 28 1972

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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