TY - JOUR
T1 - Current approaches for the analysis of spindle organization
AU - Redemann, Stefanie
AU - Fürthauer, Sebastian
AU - Shelley, Michael
AU - Müller-Reichert, Thomas
N1 - Funding Information:
Research in the Müller-Reichert lab is supported by funds from the Deutsche Forschungsgemeinschaft ( MU 1423/3-1 , 3-2 and 8-1 ) and the Human Frontier Science Programme ( RGP 0034/2010 ). S. Redemann received funding from the Faculty of Medicine Carl Gustav Carus of the TU Dresden (Frauenhabilitationsstipendium).
Publisher Copyright:
© 2019
PY - 2019/10
Y1 - 2019/10
N2 - The organization of microtubules in spindles is complex and not fully understood. Here we report on current advances in generating 3D reconstructions of staged spindles by serial-section electron tomography, exemplified by the first mitotic spindle in early Caenorhabditis elegans embryo. We then review how advances in correlative light microscopy and quantitative electron tomography enable the development of theory and stochastic simulations, which describe how the microtubule organization in spindles emerges from their dynamics. We show how theory and simulations can be used to address long-standing questions in cell division research, advancing the field beyond a pure structural description of microtubules in spindles.
AB - The organization of microtubules in spindles is complex and not fully understood. Here we report on current advances in generating 3D reconstructions of staged spindles by serial-section electron tomography, exemplified by the first mitotic spindle in early Caenorhabditis elegans embryo. We then review how advances in correlative light microscopy and quantitative electron tomography enable the development of theory and stochastic simulations, which describe how the microtubule organization in spindles emerges from their dynamics. We show how theory and simulations can be used to address long-standing questions in cell division research, advancing the field beyond a pure structural description of microtubules in spindles.
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U2 - 10.1016/j.sbi.2019.05.023
DO - 10.1016/j.sbi.2019.05.023
M3 - Review article
C2 - 31279499
AN - SCOPUS:85068259419
SN - 0959-440X
VL - 58
SP - 269
EP - 277
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -