Cyclic AMP metabolism in mouse parotid glands properties of adenylate cyclase, protein kinase and phosphodiesterase

Hua I. Chiu, Douglas J. Franks, Richard Rowe, Daniel Malamud

Research output: Contribution to journalArticle

Abstract

Catecholamines induce unique growth and secretory responses in salivary glands. An analysis of three enzyme activities involved in cyclic AMP metabolism was carried out to identify the specificity of these responses for salivary glands. Although parotid adenylate cyclase has an unusually high specific activity, its kinetic properties and responses to NaF, guanine nucleotides, and isoproterenol are similar to other tissues not stimulated to grow after isoproterenol stimulation. Solubilized adenylate cyclase was separated from other membrane proteins by isoelectric focusing on polyacrylamide gels. There was a single broad peak of activity with a pI of 5.9. Parotid protein kinase has a subcellular distribution and substrate preference similar to hepatic protein kinase. Activation by cyclic AMP is also similar to that reported for other tissues, with a Ka of 1.2·10-7 M. Parotid cyclic AMP and cyclic GMP phosphoriesterases are a heterogeneous group of enzymes with relatively low specific activity as compared with mouse pancreas, liver and brain. Isoelectric focusing of supernatant phosphodiesterases revealed at least six peaks of enzyme activity in the pI range of 4-6. Previous reports of a large increase in parotid cyclic AMP levels after in vivo administration of catecholamines and specific growth and secretion could be the result of a relatively high specific activity adenylate cyclase associated with low specific activity cyclic AMP phosphodiesterases.

Original languageEnglish (US)
Pages (from-to)29-40
Number of pages12
JournalBBA - General Subjects
Volume451
Issue number1
DOIs
StatePublished - Nov 18 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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