TY - JOUR
T1 - Deciphering the glycocode
T2 - the complexity and analytical challenge of glycomics
AU - Pilobello, Kanoelani T.
AU - Mahal, Lara K.
N1 - Funding Information:
We acknowledge the Arnold and Mabel Beckman Foundation for supporting this work.
PY - 2007/6
Y1 - 2007/6
N2 - Carbohydrates coat most types of cell in nature and are intimately involved in various biological events, including cell differentiation, homing to specific tissues, cell adhesion, cell recognition, microbial pathogenesis and immunological recognition. Carbohydrate structures are complex to analyze owing to their branched nature, the diversity of secondary modifications of monomers, their indirect relationship to the genome and the range of molecular contexts in which the modifications are found. Thus, whereas the fields of genomics and proteomics have become accessible to most scientists, technologies to assess glycan structures rapidly (i.e. glycomics) are still in the developmental stages. This review focuses on recent developments in glycomic technologies, including new high-throughput techniques for glycan purification and annotation that are advancing mass-spectrometry-based glycomics, and the latest work on microarray methodologies to decipher the glycome.
AB - Carbohydrates coat most types of cell in nature and are intimately involved in various biological events, including cell differentiation, homing to specific tissues, cell adhesion, cell recognition, microbial pathogenesis and immunological recognition. Carbohydrate structures are complex to analyze owing to their branched nature, the diversity of secondary modifications of monomers, their indirect relationship to the genome and the range of molecular contexts in which the modifications are found. Thus, whereas the fields of genomics and proteomics have become accessible to most scientists, technologies to assess glycan structures rapidly (i.e. glycomics) are still in the developmental stages. This review focuses on recent developments in glycomic technologies, including new high-throughput techniques for glycan purification and annotation that are advancing mass-spectrometry-based glycomics, and the latest work on microarray methodologies to decipher the glycome.
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U2 - 10.1016/j.cbpa.2007.05.002
DO - 10.1016/j.cbpa.2007.05.002
M3 - Review article
C2 - 17500024
AN - SCOPUS:34250693209
SN - 1367-5931
VL - 11
SP - 300
EP - 305
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 3
ER -