Decoding and perturbing decision states in real time

In dynamic environments, subjects often integrate multiple samples of a signal and combine them to reach a categorical judgment¹. The process of deliberation can be described by a time-varying decision variable (DV), decoded from neural population activity, that predicts a subject’s upcoming decision². Within single trials, however, there are large moment-to-moment fluctuations in the DV, the behavioural significance of which is unclear. Here, using real-time, neural feedback control of stimulus duration, we show that within-trial DV fluctuations, decoded from motor cortex, are tightly linked to decision state in macaques, predicting behavioural choices substantially better than the condition-averaged DV or the visual stimulus alone. Furthermore, robust changes in DV sign have the statistical regularities expected from behavioural studies of changes of mind³. Probing the decision process on single trials with weak stimulus pulses, we find evidence for time-varying absorbing decision bounds, enabling us to distinguish between specific models of decision making.